A dual-modal PET/near infrared fluorescent nanotag for long-term immune cell tracking

Adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells has emerged as a highly promising cancer therapy. The pharmacodynamic action or CAR T cells is closely related to their pharmacokinetic profile; because of this as well as the risk of non-specific action, it is important to m...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2021-02, Vol.269, p.120630-120630, Article 120630
Main Authors: Harmsen, Stefan, Medine, Emin Ilker, Moroz, Maxim, Nurili, Fuad, Lobo, Jose, Dong, Yiyu, Turkekul, Mezruh, Pillarsetty, Naga Vara Kishore, Ting, Richard, Ponomarev, Vladimir, Akin, Oguz, Aras, Omer
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cell Tracking
Dual-modal
Humans
Immune cell labeling
Immunotherapy, Adoptive
Nanomedicine
Near-infrared fluorescence
PET
Positron-Emission Tomography
Tissue Distribution
Tracking
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells has emerged as a highly promising cancer therapy. The pharmacodynamic action or CAR T cells is closely related to their pharmacokinetic profile; because of this as well as the risk of non-specific action, it is important to monitor their biodistribution and fate following infusion. To this end, we developed a dual-modal PET/near infrared fluorescent (NIRF) nanoparticle-based imaging agent for non-genomic labeling of human CAR T cells. Since the PET/NIRF nanoparticles did not affect cell viability or cytotoxic functionality and enabled long-term whole-body CAR T cell tracking using PET and NIRF in an ovarian peritoneal carcinomatosis model, this platform is a viable imaging technology to be applied in other cancer models.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2020.120630