An Injectable Fibrin Scaffold Rich in Growth Factors for Skin Repair

Platelet aggregates, such as PRP, PRF, and CGF, have been used alone or in combination with other grafting materials to enhance restoration outcomes. The process for preparing these autografting materials requires two-step centrifugation or specific centrifuges. In this study, we obtained an injecta...

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Bibliographic Details
Published in:BioMed research international 2021, Vol.2021 (1), p.8094932-8094932
Main Authors: Shao, Zhengwei, Lyu, Chengqi, Teng, Lin, Xie, Xuetao, Sun, Jiayue, Zou, Derong, Lu, Jiayu
Format: Article
Language:English
Subjects:
Analysis
Anticoagulants
Biomedical materials
Blood platelets
Blood tests
Bone marrow
Cell adhesion & migration
Cell growth
Cell proliferation
Centrifugation
Collagen
Controlled release
Cultures (Biology)
Deposition
Enzyme-linked immunosorbent assay
Enzymes
Extracellular matrix
Fibrin
Fibroblast growth factors
Fibroblasts
Growth factors
Infections
Insulin-like growth factors
Leukocytes
Medical research
Medicine, Experimental
Methods
Morphology
Patient outcomes
Penicillin
Platelet-derived growth factor
Platelets
Porosity
Rabbits
Repair
Scaffolds
Scanning electron microscopy
Skin
Skin-grafting
Sustained release
Tissue engineering
Transforming growth factor-b1
Vascular endothelial growth factor
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Summary:Platelet aggregates, such as PRP, PRF, and CGF, have been used alone or in combination with other grafting materials to enhance restoration outcomes. The process for preparing these autografting materials requires two-step centrifugation or specific centrifuges. In this study, we obtained an injectable fibrin scaffold (IFS) rich in growth factors by one-step centrifugation of whole blood from rabbits. The purpose of this study is to introduce some characteristics of IFS. This scaffold was characterized using various techniques, including Masson’s trichrome staining, scanning electron microscopy, porosity measurements, and cell counting. The sustained release of growth factors, including PDGF, VEGF, TGF-β1, IGF, FGF, and EGF, was quantified using ELISA assay. The obtained IFS was tested for its effects on cell proliferation, extracellular matrix deposition, and full-thickness skin defect repair. The prepared IFS is characterized by a loose fibrin network structure with white blood cells and platelets that slowly release growth factors and can promote the healing of skin defects via the promotion of cell proliferation, collagen deposition, and tissue revascularization. In addition, its liquid properties and porous structure are conducive to its application as a therapeutic component in tissue engineering.
ISSN:2314-6133
2314-6141
DOI:10.1155/2021/8094932