Tolerance to tomato yellow leaf curl virus in transgenic tomato overexpressing a cellulose synthase‐like gene

A recent study in tomato identified 38 cellulose synthesis genes with in this gene family, including 16 cellulose synthase (CesA) and 22 cellulose synthase‐like (Csl) genes (Song et al., 2019). [...]we re‐examined our previously reported RNA‐seq data set obtained by transcriptomic analysis in health...

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Bibliographic Details
Published in:Plant biotechnology journal 2021-04, Vol.19 (4), p.657-659
Main Authors: Choe, Siwon, Choi, Boram, Kang, Jin‐Ho, Seo, Jang‐Kyun
Format: Article
Language:English
Subjects:
Begomovirus - genetics
Biosynthesis
Brief Communication
Brief Communications
Cellulose
Cellulose synthase
cellulose synthase‐like genes
cellulose synthesis
Conflicts of interest
Crop diseases
disease tolerance
Experiments
Flowers & plants
Gene expression
Gene silencing
Genes
Glucosyltransferases - genetics
Infections
Leaf-curl
Lycopersicon esculentum - genetics
Metabolism
Pathogens
Phenotypes
Plant Diseases
Plant virus diseases
Productivity
symptom suppression
Synthesis
Tobacco
Tomatoes
Transcriptomics
TYLCV
Variance analysis
Viral infections
Viruses
Yellow leaf
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Summary:A recent study in tomato identified 38 cellulose synthesis genes with in this gene family, including 16 cellulose synthase (CesA) and 22 cellulose synthase‐like (Csl) genes (Song et al., 2019). [...]we re‐examined our previously reported RNA‐seq data set obtained by transcriptomic analysis in healthy and TYLCV‐infected tomato plants (Seo et al., 2018), focusing on the 38 CesA/Csl genes. Different letters above the bars indicate significant differences between samples (one‐way ANOVA, P < 0.05). Since no functional characterization of cellulose synthesis genes has been done so far in tomato, we first examined the loss‐of‐function phenotypes associated with the differential expression of five CesA/Csl genes upon TYLCV infection by employing a tobacco rattle virus (TRV)‐based virus‐induced gene silencing (VIGS) system (Liu et al., 2002). Solyc07g043390 (Csl) and Solyc12g056580 (CesA) silencing caused strong growth inhibition in tomato plants, whereas no distinguishable phenotypic differences were observed when the other three Csl genes (Solyc07g051820, Solyc08g076320, and Solyc03g097050) were silenced (Figure 1d). Since TYLCV infection caused dramatic down‐regulation of Solyc07g043390, and its silencing resulted in a stunted growth phenotype, a representative symptom of TYLCV infection, we further focused on the functional characterization of this gene.
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.13539