Characterization of the SARS-CoV-2 coronavirus X4-like accessory protein

• Published in: Egyptian Journal of Medical Human Genetics 2021-05, Vol.22 (1), p.48-11
• Main Authors: Durojaye, Olanrewaju Ayodeji, Okoro, Nkwachukwu Oziamara, Odiba, Arome Solomon
• Format: Article
• Language: English
• Subjects: Conserved sequence; Coronavirus; Coronaviruses; COVID-19; Pharmaceutical industry; Phylogeny; Proteins; SARS-CoV-2; Sequence analysis; Severe acute respiratory syndrome coronavirus 2; Vaccines; X4 protein
• ISSN: 1110-8630; 2090-2441
• DOI: 10.1186/s43042-021-00160-1

Background The novel coronavirus SARS-CoV-2 is currently a global threat to health and economies. Therapeutics and vaccines are in rapid development; however, none of these therapeutics are considered as absolute cure, and the potential to mutate makes it necessary to find therapeutics that target a highly conserved regions of the viral structure. Results In this study, we characterized an essential but poorly understood coronavirus accessory X4 protein, a core and stable component of the SARS-CoV family. Sequence analysis shows a conserved ~ 90% identity between the SARS-CoV-2 and previously characterized X4 protein in the database. QMEAN Z score of the model protein shows a value of around 0.5, within the acceptable range 0-1. A MolProbity score of 2.96 was obtained for the model protein and indicates a good quality model. The model has Ramachandran values of [phi] = - 57.sup.o and [psi] = - 47.sup.o for [alpha]-helices and values of [phi] = - 130.sup.o and [psi] = + 140.sup.o for twisted sheets. Conclusions The protein data obtained from this study provides robust information for further in vitro and in vivo experiment, targeted at devising therapeutics against the virus. Phylogenetic analysis further supports previous evidence that the SARS-CoV-2 is positioned with the SL-CoVZC45, BtRs-BetaCoV/YN2018B and the RS4231 Bat SARS-like corona viruses.