Novel IM‐associated protein Tim54 plays a role in the mitochondrial import of internal signal‐containing proteins in Trypanosoma brucei

• Published in: Biology of the cell 2021-01, Vol.113 (1), p.39-57
• Main Authors: Singha, Ujjal K., Tripathi, Anuj, Smith, Joseph T., Quinones, Linda, Saha, Aparajita, Singha, Tanusree, Chaudhuri, Minu
• Format: Article
• Language: English
• Subjects: Mitochondrial; TbTim17; TbTim54; Translocase; Trypanosoma brucei
• ISSN: 0248-4900; 1768-322X
• DOI: 10.1111/boc.202000054

Background The translocase of the mitochondrial inner membrane (TIM) imports most of the nucleus‐encoded proteins that are destined for the matrix, inner membrane (IM) and the intermembrane space (IMS). Trypanosoma brucei, the infectious agent for African trypanosomiasis, possesses a unique TIM complex consisting of several novel proteins in association with a relatively conserved protein TbTim17. Tandem affinity purification of the TbTim17 protein complex revealed TbTim54 as a potential component of this complex. Results TbTim54, a trypanosome‐specific IMS protein, is peripherally associated with the IM and is present in a protein complex slightly larger than the TbTim17 complex. TbTim54 knockdown (KD) reduced the import of TbTim17 and compromised the integrity of the TbTim17 complex. TbTim54 KD inhibited the in vitro mitochondrial import and assembly of the internal signal‐containing mitochondrial carrier proteins MCP3, MCP5 and MCP11 to a greater extent than TbTim17 KD. Furthermore, TbTim54 KD, but not TbTim17 KD, significantly hampered the mitochondrial targeting of ectopically expressed MCP3 and MCP11. These observations along with our previous finding that the mitochondrial import of N‐terminal signal‐containing proteins like cytochrome oxidase subunit 4 and MRP2 was affected to a greater extent by TbTim17 KD than TbTim54 KD indicating a substrate‐specificity of TbTim54 for internal‐signal containing mitochondrial proteins. In other organisms, small Tim chaperones in the IMS are known to participate in the translocation of MCPs. We found that TbTim54 can directly interact with at least two of the six known small TbTim proteins, TbTim11 and TbTim13, as well as with the N‐terminal domain of TbTim17. Conclusion TbTim54 interacts with TbTim17. It also plays a crucial role in the mitochondrial import and complex assembly of internal signal‐containing IM proteins in T. brucei. Significance We are the first to characterise TbTim54, a novel TbTim that is involved primarily in the mitochondrial import of MCPs and TbTim17 in T. brucei. Research article: Trypanosoma brucei, the infectious agent for African trypanosomiasis, imports hundreds of nuclear‐encoded proteins through the mitochondrial inner membrane (IM) via a unique TIM complex consisting of several novel proteins in association with a relatively conserved protein TbTim17. TbTim54, a novel peripherally associated IM protein, associates with TbTim17 and small TbTims and plays an important role in mitoch