Development and Validation of a Multiplex Microsphere Immunoassay Using Dried Blood Spots for SARS-CoV-2 Seroprevalence: Application in First Responders in Colorado, USA

Serological testing of large representative populations for antibodies to SARS-CoV-2 is needed to estimate seroprevalence, transmission dynamics, and the duration of antibody responses from natural infection and vaccination. In this study, a high-throughput SARS-CoV-2 multiplex microsphere immunoass...

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Bibliographic Details
Published in:Journal of clinical microbiology 2021-05, Vol.59 (6)
Main Authors: Schultz, Jonathan S, McCarthy, Mary K, Rester, Cody, Sabourin, Katherine R, Annen, Kyle, DomBourian, Melkon, Eisenmesser, Elan, Frazer-Abel, Ashley, Knight, Vijaya, Jaenisch, Thomas, Morrison, Thomas E, Rochford, Rosemary, Kedl, Ross M
Format: Article
Language:English
Subjects:
Antibodies, Viral
Colorado
COVID-19
COVID-19 Serological Testing
Emergency Responders
Humans
Immunoassay
Immunoassays
Microspheres
SARS-CoV-2
Seroepidemiologic Studies
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Summary:Serological testing of large representative populations for antibodies to SARS-CoV-2 is needed to estimate seroprevalence, transmission dynamics, and the duration of antibody responses from natural infection and vaccination. In this study, a high-throughput SARS-CoV-2 multiplex microsphere immunoassay (MMIA) was developed for the receptor binding domain (RBD) and nucleocapsid (N) that was more sensitive than enzyme-linked immunosorbent assay (ELISA) (98% versus 87%). The MMIA was then applied and validated in 264 first responders in Colorado using serum and dried blood spot (DBS) eluates, compared to ELISA, and evaluated for neutralizing antibodies. Four percent (11/264) of first responders were seropositive in July to August 2020. Serum and DBS were highly correlated for anti-RBD and anti-N antibodies (  = 0.83,  
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00290-21