Cytomegalovirus Glycoprotein Polymorphisms and Increasing Viral Load in Non-Transplant Patients with Hematological Malignancies Undergoing Chemotherapy: A Prospective Observational Study

Introduction Cytomegalovirus (CMV) predisposes to several clinical complications and is a major cause of morbidity and mortality in immunocompromised patients, including patients with hematological malignancies (HM). The present study was carried out to determine the distribution of CMV glycoprotein...

Full description

Saved in:
Bibliographic Details
Published in:Infectious diseases and therapy 2021-09, Vol.10 (3), p.1549-1566
Main Authors: Handous, Imene, Hannachi, Naila, Achour, Bechir, Marzouk, Manel, Hazgui, Olfa, Khelif, Abderrahim, Boukadida, Jalel
Format: Article
Language:English
Subjects:
Analysis
Cancer
Chemotherapy
Cytomegalovirus
Genetic aspects
Genotype & phenotype
Genotypes
Glycoproteins
Health aspects
Hematology
Hematopoietic stem cells
Infectious Diseases
Internal Medicine
Leukemia
Load distribution
Medicine
Medicine & Public Health
Mortality
Observational studies
Original Research
Skin diseases
Stem cells
Transplantation
Tunisia
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Introduction Cytomegalovirus (CMV) predisposes to several clinical complications and is a major cause of morbidity and mortality in immunocompromised patients, including patients with hematological malignancies (HM). The present study was carried out to determine the distribution of CMV glycoprotein B, N, and O (gB, gN, and gO) genotypes and their potential effect on its viral load and on clinical outcomes in a cohort of Tunisian non-hematopoietic stem cell transplant (HSCT) patients with HM undergoing chemotherapy. Methods CMV viral load was evaluated by real-time quantitative PCR. The gB, gN, and gO genotypes of the CMV strains were analyzed by multiplex nested PCR and sequencing. Results This prospective study involved 60 clinical isolates obtained from 60 non-HSCT patients with HM undergoing chemotherapy. Mixed CMV gB, gN, and gO genotypes were the predominant glycoprotein genotypes in 31%, 41.4%, and 46.4% of patients, respectively. Mixed gB genotypes were associated with higher initial levels of CMV load ( p  = 0.001), increased rate of fever (0.025), and co-infection with other herpesviruses (HHVs) ( p  = 0.024) more frequently than in single gB genotype. Mixed gN genotypes were more associated with severe lymphopenia (ALC 
ISSN:2193-8229
2193-6382
DOI:10.1007/s40121-021-00457-z