Combining Sandblasting, Alkaline Etching, and Collagen Immobilization to Promote Cell Growth on Biomedical Titanium Implants

Our objective in this study was to promote the growth of bone cells on biomedical titanium (Ti) implant surfaces via surface modification involving sandblasting, alkaline etching, and type I collagen immobilization using the natural cross-linker genipin. The resulting surface was characterized in te...

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Bibliographic Details
Published in:Polymers 2021-07, Vol.13 (15), p.2550
Main Authors: Liu, Chia-Fei, Chang, Kai-Chun, Sun, Ying-Sui, Nguyen, Diem Thuy, Huang, Her-Hsiung
Format: Article
Language:English
Subjects:
Biocompatibility
Bone marrow
Cell adhesion
Cell adhesion & migration
Cell growth
Collagen
Contact angle
Differentiation (biology)
Etching
Field emission microscopy
Fourier transforms
Functional groups
Genipin
Immobilization
Infrared reflection
Mineralization
Morphology
Proteins
Sample size
Sandblasting
Shear strength
Stem cells
Surface properties
Surface roughness
Surface treatment
Surgical implants
Titanium
Transplants & implants
Variance analysis
Wettability
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Summary:Our objective in this study was to promote the growth of bone cells on biomedical titanium (Ti) implant surfaces via surface modification involving sandblasting, alkaline etching, and type I collagen immobilization using the natural cross-linker genipin. The resulting surface was characterized in terms topography, roughness, wettability, and functional groups, respectively using field emission scanning electron microscopy, 3D profilometry, and attenuated total reflection-Fourier transform infrared spectroscopy. We then evaluated the adhesion, proliferation, initial differentiation, and mineralization of human bone marrow mesenchymal stem cells (hMSCs). Results show that sandblasting treatment greatly enhanced surface roughness to promote cell adhesion and proliferation and that the immobilization of type I collagen using genipin enhanced initial cell differentiation as well as mineralization in the extracellular matrix of hMSCs. Interestingly, the nano/submicro-scale pore network and/or hydrophilic features on sandblasted rough Ti surfaces were insufficient to promote cell growth. However, the combination of all proposed surface treatments produced ideal surface characteristics suited to Ti implant applications.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym13152550