Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

The aim of the study was to investigate specific potential markers for cells obtained from three layers of human AAA divided into three segments along the AAA based on morphological differences. The isolated cells were compared to control commercial cell types from healthy human abdominal aortas. Fo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied genetics 2021-09, Vol.62 (3), p.487-497
Main Authors: Lesiak, Marta, Augusciak-Duma, Aleksandra, Stepien, Karolina L., Fus-Kujawa, Agnieszka, Botor, Malwina, Sieron, Aleksander L.
Format: Article
Language:English
Subjects:
Analysis
Aneurysms
Animal Genetics and Genomics
Antibodies
Aorta
Aortic aneurysms
Biomarkers
Biomedical and Life Sciences
Cell culture
Computer software industry
Flow cytometry
Gene expression
Gene flow
Genes
Human Genetics
Human Genetics • Original Paper
Life Sciences
Microbial Genetics and Genomics
Plant Genetics and Genomics
Statistical analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the study was to investigate specific potential markers for cells obtained from three layers of human AAA divided into three segments along the AAA based on morphological differences. The isolated cells were compared to control commercial cell types from healthy human abdominal aortas. For each type of aortic layer, three specimens from 6 patients were compared. Total RNA was isolated from 36 cell cultures for gene expression profiling and potential new cytometry markers were typed. Isolated cells were analyzed by flow cytometry by using fluorochrome-conjugated antibodies to markers: CNN1, MYH10, ENG, ICAM2, and TEK. The relative expression of 45 genes in primary cell cultures and control lines was analyzed. Statistically significant differences were found in the expression of most of the analyzed genes between individual layers and control lines. Based on relative expression, antibodies were selected for flow cytometry. Gene expression profiles allowed to select new potential cytometry markers: CNN1, MYH10, MYOCD, ENG, ICAM2, TEK. However, none of the tested markers seems to be optimal and characteristic for a specific layer of AAA.
ISSN:1234-1983
2190-3883
DOI:10.1007/s13353-021-00641-4