Death From Primary Amebic Meningoencephalitis After Recreational Water Exposure During Recent Travel to India—Santa Clara County, California, 2020

• Published in: Open forum infectious diseases 2021-08, Vol.8 (8), p.ofab322-ofab322
• Main Authors: Harris, Glenn R, Karmarkar, Ellora N, Quenelle, Rebecca, Chaille, Lyndsey, Madhok, Jai, Tien, Vivian, Gupta, Jyoti, Jain, Seema, Liu, Maria, Roy, Shantanu, Narasimhan, Supriya, Kimura, Akiko, Cope, Jennifer R, Ali, Ibne Karim M
• Format: Article
• Language: English
• Subjects: Editor's Choice; Major
• ISSN: 2328-8957; 2328-8957
• DOI: 10.1093/ofid/ofab322

Abstract Background In February 2020, a man returned to the United States after an 11-day trip to India and died of primary amebic meningoencephalitis (PAM), caused by nasal exposure to the free-living ameba Naegleria fowleri found in warm water. We identified potential exposures, confirmed etiology, and described the molecular epidemiology of the infection. Methods We reviewed medical records to describe his clinical course and interviewed his family to determine water exposures. Genotyping was performed on the N. fowleri strain and compared with North American strains through repetitive nonpolymorphic nuclear loci analysis to identify differences. We reviewed N. fowleri strains in the National Center for Biotechnology Information database (GenBank) to determine genotypes present in India. Results The patient became acutely encephalopathic 3 days after returning; the only known nasal water exposure was at an indoor swimming pool in India 5 days earlier. Cerebrospinal fluid (CSF) testing demonstrated neutrophil-predominant pleocytosis and low glucose, but negative gram stain and culture. CSF microscopy revealed trophozoites; N. fowleri was detected by real-time polymerase chain reaction. Classical genotyping confirmed genotype I, common in the United States and among Indian strains in GenBank. The North American N. fowleri strains and the patient’s strain varied at 5 nonpolymorphic loci. Conclusions A man died from PAM after likely exposure at a vacation rental pool in India. We recommend including PAM in the differential diagnosis when CSF studies suggest bacterial meningitis but gram stain is negative. Genotyping can advance our understanding of N. fowleri molecular epidemiology and support future investigations. After traveling to India, a man from the United States developed acute encephalopathy, was diagnosed with primary amebic meningoencephalitis from Naegleria fowleri infection, and died. His exposure history and genetic analysis of the N. fowleri isolate suggest infection occurred abroad.