Serum and Tissue Level of TLR9 in EBV-Associated Oropharyngeal Cancer

The Epstein–Barr virus (EBV) is associated with the development of various epithelial malignancies including cancer in the head and neck region. Several studies have shown that Toll-like receptors (TLRs) are required for an innate immune response to infection with human DNA viruses, e.g., EBV. Durin...

Full description

Saved in:
Bibliographic Details
Published in:Cancers 2021-08, Vol.13 (16), p.3981
Main Authors: Stępień, Ewa, Strycharz-Dudziak, Małgorzata, Malm, Maria, Drop, Bartłomiej, Polz-Dacewicz, Małgorzata
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Autoantigens
Cancer
Cytokines
Deoxyribonucleic acid
DNA
DNA polymerase
DNA viruses
Enzymes
Epstein-Barr virus
Head & neck cancer
Immune response
Immunology
Infections
Innate immunity
Interleukin 10
Lymph nodes
Lymphoma
Malignancy
Oropharyngeal cancer
Pathogens
Patients
Polymerase chain reaction
Serology
TLR9 protein
Toll-like receptors
Tumor necrosis factor-α
Tumors
Vascular endothelial growth factor
Viral infections
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Epstein–Barr virus (EBV) is associated with the development of various epithelial malignancies including cancer in the head and neck region. Several studies have shown that Toll-like receptors (TLRs) are required for an innate immune response to infection with human DNA viruses, e.g., EBV. During viral infections, TLR response may influence the transformation to malignancy. The aim of the study was to assess TLR9 serum and tissue level in EBV(+) and EBV(−) oropharyngeal cancer patients. The study involved 78 patients: 42 EBV(+) and 36 EBV(−). EBV DNA was detected in fresh frozen tumor tissue. TLR9 level was measured in homogenate of tumor tissue and in serum. Moreover, in serum samples IL-10, VEGF, TGFβ, TNFα and antibodies against EBV were detected using ELISA test. TLR9 level was significantly lower in EBV(+) patients, both in tissue and serum, while EBVCA, EBNA and VEGF level was statistically higher in EBV(+) patients. An increase in EBVCA and EBNA antibodies titer was correlated with a TLR9 level decrease. TLR9 level was higher in poorly-differentiated tumors (G3), in tumor of larger dimensions (T3-T4) and with lymph nodes involvement (N3-N4) but without statistical significance. High levels of anti-EA antibodies in the majority of EBV(+) patients may point to the reactivation of EBV infection.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers13163981