In Silico Study, Physicochemical, and In Vitro Lipase Inhibitory Activity of α , β -Amyrenone Inclusion Complexes with Cyclodextrins

• Published in: International journal of molecular sciences 2021-09, Vol.22 (18), p.9882
• Main Authors: Oliveira, Luana Carvalho de, Menezes, Danielle Lima Bezerra de, Silva, Valéria Costa da, Lourenço, Estela Mariana Guimarães, Miranda, Paulo Henrique Santana, Silva, Márcia de Jesus Amazonas da, Lima, Emerson Silva, Júnior, Valdir Florêncio da Veiga, Marreto, Ricardo Neves, Converti, Attilio, Barbosa, Euzébio Guimaraes, Lima, Ádley Antonini Neves de
• Format: Article
• Language: English
• Subjects: Animals; beta-Cyclodextrins - chemistry; beta-Cyclodextrins - pharmacology; Bioavailability; Calorimetry, Differential Scanning; Computer Simulation; Cyclodextrin; Cyclodextrins; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; Enzymes; Inclusion complexes; Lipase; Lipase - antagonists & inhibitors; Lipase - chemistry; Modelling; Molecular modelling; Obesity; Orlistat - pharmacology; Physicochemical properties; Solubility - drug effects; Spectroscopy, Fourier Transform Infrared; Swine; Triterpenes - chemical synthesis; Triterpenes - chemistry; Triterpenes - pharmacology; Weight control; X-Ray Diffraction
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22189882

, -amyrenone (ABAME) is a triterpene derivative with many biological activities; however, its potential pharmacological use is hindered by its low solubility in water. In this context, the present work aimed to develop inclusion complexes (ICs) of ABAME with - and -cyclodextrins (CD), which were systematically characterized through molecular modeling studies as well as FTIR, XRD, DSC, TGA, and SEM analyses. In vitro analyses of lipase activity were performed to evaluate possible anti-obesity properties. Molecular modeling studies indicated that the CD:ABAME ICs prepared at a 2:1 molar ratio would be more stable to the complexation process than those prepared at a 1:1 molar ratio. The physicochemical characterization showed strong evidence that corroborates with the in silico results, and the formation of ICs with CD was capable of inducing changes in ABAME physicochemical properties. ICs was shown to be a stronger inhibitor of lipase activity than Orlistat and to potentiate the inhibitory effects of ABAME on porcine pancreatic enzymes. In conclusion, a new pharmaceutical preparation with potentially improved physicochemical characteristics and inhibitory activity toward lipases was developed in this study, which could prove to be a promising ingredient for future formulations.