Comparison of Microneutralization and Histo-Blood Group Antigen–Blocking Assays for Functional Norovirus Antibody Detection

Abstract Background The development of an in vitro cultivation system for human noroviruses allows the measurement of neutralizing antibody levels. Methods Serum neutralizing antibody levels were determined using a GII.4/Sydney/2012-like virus in human intestinal enteroids in samples collected befor...

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Bibliographic Details
Published in:The Journal of infectious diseases 2020-02, Vol.221 (5), p.739-743
Main Authors: Atmar, Robert L, Ettayebi, Khalil, Ayyar, B Vijayalakshmi, Neill, Frederick H, Braun, Ralph P, Ramani, Sasirekha, Estes, Mary K
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antibodies
Antibodies, Blocking - immunology
Antibodies, Neutralizing - immunology
Antibodies, Viral - analysis
Antibodies, Viral - immunology
Antigens
Blocking antibodies
Blood Group Antigens - immunology
Blood groups
Caliciviridae Infections - prevention & control
Caliciviridae Infections - virology
Clinical trials
Female
Healthy Volunteers
Humans
Intestine
Major and Brief Reports
Male
Middle Aged
Neutralization Tests
Norovirus - immunology
Vaccination
Viral Vaccines - immunology
Young Adult
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Summary:Abstract Background The development of an in vitro cultivation system for human noroviruses allows the measurement of neutralizing antibody levels. Methods Serum neutralizing antibody levels were determined using a GII.4/Sydney/2012-like virus in human intestinal enteroids in samples collected before and 4 weeks after administration of an investigational norovirus vaccine and were compared with those measured in histo-blood group antigen (HBGA)–blocking assays. Results Neutralizing antibody seroresponses were observed in 71% of 24 vaccinated adults, and antibody levels were highly correlated (r = 0.82, P < .001) with those measured by HBGA blocking. Conclusions HBGA-blocking antibodies are a surrogate for neutralization in human noroviruses. Clinical Trials Registration NCT02475278. Norovirus antibody levels measured by microneutralization are highly correlated with those measured by histo-blood group antigen–blocking assays.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jiz526