Highly specific and rapid molecular detection of Candida glabrata in clinical samples

The most common nosocomial fungal infections are caused by several species of Candida , of which Candida glabrata is the second most frequently isolated species from bloodstream infections. C. glabrata displays relatively high minimal inhibitory concentration values (MIC) to the antifungal fluconazo...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2021-12, Vol.52 (4), p.1733-1744
Main Authors: Hernández-Carreón, Oscar, Hernández-Howell, Cesia, Hernández-Hernández, Grecia, Herrera-Basurto, M. Selene, González-Gómez, Blanca E., Gutiérrez-Escobedo, Guadalupe, García-Calderón, Norma I., Barrón-Pastor, Daniel, De Las Peñas, Alejandro, Castaño, Irene
Format: Article
Language:English
Subjects:
Amplification
Antifungal Agents - pharmacology
Antifungal Agents - therapeutic use
Bacterial
Bacterial, Fungal and Virus Molecular Biology - Research Paper
Biomedical and Life Sciences
Candida
Candida glabrata
Candida glabrata - drug effects
Candida glabrata - genetics
Candidiasis - diagnosis
Candidiasis - microbiology
Deoxyribonucleic acid
DNA
DNA Primers
Fluconazole
Fluconazole - pharmacology
Food Microbiology
Fungal and Virus Molecular Biology - Research Paper
Fungicides
Genomes
Hospitals
Humans
Infections
Life Sciences
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbial Sensitivity Tests
Microbiology
Minimum inhibitory concentration
Mortality
Mycology
Nosocomial infection
Polymerase Chain Reaction
Species
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Summary:The most common nosocomial fungal infections are caused by several species of Candida , of which Candida glabrata is the second most frequently isolated species from bloodstream infections. C. glabrata displays relatively high minimal inhibitory concentration values (MIC) to the antifungal fluconazole and is associated with high mortality rates. To decrease mortality rates, the appropriate treatment must be administered promptly. C. glabrata contains in its genome several non-identical copies of species-specific sequences. We designed three pairs of C. glabrata -specific primers for endpoint PCR amplification that align to these species-specific sequences and amplify the different copies in the genome. Using these primers, we developed a fast, sensitive, inexpensive, and highly specific PCR-based method to positively detect C. glabrata DNA in a concentration-dependent manner from mixes of purified genomic DNA of several Candida species, as well as from hemocultures and urine clinical samples. This tool can be used for positive identification of C. glabrata in the clinic.
ISSN:1517-8382
1678-4405
DOI:10.1007/s42770-021-00584-2