Comparative RNA-Seq Analysis Reveals Potentially Resistance-Related Genes in Response to Bacterial Canker of Tomato

• Published in: Genes 2021-10, Vol.12 (11), p.1745
• Main Authors: Pereyra-Bistraín, Leonardo I, Ovando-Vázquez, Cesaré, Rougon-Cardoso, Alejandra, Alpuche-Solís, Ángel G
• Format: Article
• Language: English
• Subjects: Ankyrins; Bacteria; Bacterial Infections - microbiology; Canker; Clavibacter; Clavibacter michiganensis michiganensis; Disease Resistance - genetics; Domestication; Flowers & plants; Gene expression; Gene Expression Regulation, Plant; Gene mapping; Gene Ontology; Genetic engineering; Genome, Plant; Genomes; Glycerol; Glycerol kinase; Host-Pathogen Interactions; Infections; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; Lycopersicon esculentum - microbiology; Phenotype; Phenotypes; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Polyphenol oxidase; RNA-Seq; Solanum arcanum; Solanum lycopersicum; Species; Tomatoes; Transcription; Transcriptome; Transcriptomes
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes12111745

Tomato is one of the most important crops for human consumption. Its production is affected by the actinomycete subsp. ( ), one of the most devastating bacterial pathogens of this crop. Several wild tomato species represent a source of natural resistance to . Here, we contrasted the transcriptomes of the resistant wild tomato species LA2157 and the susceptible species cv. Ailsa Craig, during the first 24 h of challenge with . We used three analyses approaches which demonstrated to be complementary: mapping to reference genome SL3.0; semi de novo transcriptome assembly; and de novo transcriptome assembly. In a global context, transcriptional changes seem to be similar between both species, although there are some specific genes only upregulated in during interaction, suggesting that the resistance regulatory mechanism probably diverged during the domestication process. Although showed enriched functional groups related to defense, displayed a higher number of induced genes related to bacterial, oomycete, and fungal defense at the first few hours of interaction. This study revealed genes that may contribute to the resistance phenotype in the wild tomato species, such as those that encode for a polyphenol oxidase E, diacyl glycerol kinase, TOM1-like protein 6, and an ankyrin repeat-containing protein, among others. This work will contribute to a better understanding of the defense mechanism against , and the development of new control methods.