Soluble factors of mesenchimal stem cells (FS-MSC) as a potential tool to reduce inflammation in donor's lungs after hypovolemic shock

The shortage of viable lungs is still a major obstacle for transplantation. Trauma victims who represent potential lung donors commonly present hypovolemic shock leading to pulmonary inflammation and deterioration and rejection after transplantation. Seeking to improve lung graft, new approaches to...

Full description

Saved in:
Bibliographic Details
Published in:Jornal brasileiro de pneumologia 2021-01, Vol.47 (4), p.e20200452-e20200452
Main Authors: Dias, Vinicius Luderer, Braga, Karina Andrighetti de Oliveira, Nepomuceno, Natalia Aparecida, Ruiz, Liliane Moreira, Perez, Juan David Ruiz, Correia, Aristides Tadeu, Caires Junior, Luiz Carlos de, Goulart, Ernesto, Zatz, Mayana, Pêgo-Fernandes, Paulo Manuel
Format: Article
Language:English
Subjects:
Animals
Disease Models, Animal
Inflammation
Lung
Lung Transplantation
Mesenchymal Stem Cells
Original
Rats
Shock, Hemorrhagic - therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The shortage of viable lungs is still a major obstacle for transplantation. Trauma victims who represent potential lung donors commonly present hypovolemic shock leading to pulmonary inflammation and deterioration and rejection after transplantation. Seeking to improve lung graft, new approaches to donor treatment have been tested. This study focuses on treatment with mesenchymal stem cells (MSCs) or soluble factors produced by MSCs (FS-MSC) using a rat model for lung donors after hemorrhagic shock. Forty-eight rats were divided into four groups: Sham (n=12), animals without induction of hypovolemic shock; Shock (n=12), animals submitted to hypovolemic shock (mean arterial pressure 40 mmHg); MSC (n=12), animals submitted to hypovolemic shock and treated with MSCs, and FS (n=12), animals submitted to hypovolemic shock and treated with FS-MSC. The animals were subjected to a 50-minute hypovolemic shock (40 mmHg) procedure. The treated animals were monitored for 115 minutes. We performed histopathology of lung tissue and quantification of inflammatory markers (TNF-α, IL-1β, IL-6, IL-10, iCAM and vCAM) in lung tissue and peripheral blood leukocytes (PBLs). Hemorrhagic shock resulted in higher PBLs and neutrophil infiltrate in the lungs. FS animals had lower neutrophil density comparing with Shock and MSC animals (p
ISSN:1806-3756
1806-3713
1806-3756
DOI:10.36416/1806-3756/e20200452