Bacterial genomic epidemiology with mixed samples

Genomic epidemiology is a tool for tracing transmission of pathogens based on whole-genome sequencing. We introduce the mGEMS pipeline for genomic epidemiology with plate sweeps representing mixed samples of a target pathogen, opening the possibility to sequence all colonies on selective plates with...

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Bibliographic Details
Published in:Microbial genomics 2021-11, Vol.7 (11)
Main Authors: Mäklin, Tommi, Kallonen, Teemu, Alanko, Jarno, Samuelsen, Ørjan, Hegstad, Kristin, Mäkinen, Veli, Corander, Jukka, Heinz, Eva, Honkela, Antti
Format: Article
Language:English
Subjects:
Genome, Bacterial
Genomics
Methods
Sequence Analysis
Whole Genome Sequencing
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Summary:Genomic epidemiology is a tool for tracing transmission of pathogens based on whole-genome sequencing. We introduce the mGEMS pipeline for genomic epidemiology with plate sweeps representing mixed samples of a target pathogen, opening the possibility to sequence all colonies on selective plates with a single DNA extraction and sequencing step. The pipeline includes the novel mGEMS read binner for probabilistic assignments of sequencing reads, and the scalable pseudoaligner Themisto. We demonstrate the effectiveness of our approach using closely related samples in a nosocomial setting, obtaining results that are comparable to those based on single-colony picks. Our results lend firm support to more widespread consideration of genomic epidemiology with mixed infection samples.
ISSN:2057-5858
2057-5858
DOI:10.1099/mgen.0.000691