Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections

Uropathogenic (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approac...

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Bibliographic Details
Published in:Microbial genomics 2021-12, Vol.7 (12)
Main Authors: García, Vanesa, Grønnemose, Rasmus B, Torres-Puig, Sergi, Kudirkiene, Egle, Piantelli, Mateo, Ahmed, Shahana, Andersen, Thomas E, Møller-Jensen, Jakob, Olsen, John E, Herrero-Fresno, Ana
Format: Article
Language:English
Subjects:
Animals
Bacteriological Techniques
Bacteriuria - microbiology
Culture Media - chemistry
Cystitis - microbiology
Escherichia coli Proteins - genetics
Gene Expression Regulation, Bacterial - drug effects
Genes, Essential
Genetic Fitness
Glucose - administration & dosage
Glucose - chemistry
Glucose - pharmacology
High-Throughput Nucleotide Sequencing
Humans
Mice
Mutagenesis, Insertional
Sequence Analysis, DNA - methods
Uropathogenic Escherichia coli - classification
Uropathogenic Escherichia coli - genetics
Uropathogenic Escherichia coli - growth & development
Virulence Factors - genetics
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Summary:Uropathogenic (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approach (TraDIS) to reveal the UTI89 essential-genes for growth and fitness-gene-sets for growth in Luria broth (LB) and EZ-MOPS medium without glucose, as well as for human bacteriuria and mouse cystitis. A total of 293 essential genes for growth were identified and the set of fitness-genes was shown to differ depending on the growth media. A modified, previously validated UTI murine model, with administration of glucose prior to infection was applied. Selected fitness-genes for growth in urine and mouse-bladder colonization were validated using deletion-mutants. Novel fitness-genes, such as and involved in sulphur-acquisition, magnesium-uptake, and LPS-biosynthesis, were proved to be important during UTI. Moreover, was confirmed as relevant in both niches, and therefore it may represent a target for novel UTI-treatment/prevention strategies.
ISSN:2057-5858
2057-5858
DOI:10.1099/mgen.0.000719