Three Microbial Musketeers of the Seas: Shewanella baltica , Aliivibrio fischeri and Vibrio harveyi , and Their Adaptation to Different Salinity Probed by a Proteomic Approach

• Published in: International journal of molecular sciences 2022-01, Vol.23 (2), p.619
• Main Authors: Kloska, Anna, Cech, Grzegorz M, Nowicki, Dariusz, Maciąg-Dorszyńska, Monika, Bogucka, Aleksandra E, Markert, Stephanie, Becher, Dörte, Potrykus, Katarzyna, Czaplewska, Paulina, Szalewska-Pałasz, Agnieszka
• Format: Article
• Language: English
• Subjects: Adaptation; Adaptation, Physiological - genetics; Aliivibrio fischeri; Aliivibrio fischeri - genetics; Aliivibrio fischeri - metabolism; Aliivibrio fischeri - physiology; Bacteria; Bacterial Proteins - metabolism; Biological activity; Deoxyribonucleic acid; DNA; DNA-binding protein; Environmental conditions; Gene expression; Gene Expression Regulation, Bacterial; Gene Ontology; Gene regulation; Habitats; Liquid chromatography; Marine environment; Marine microorganisms; Mass spectrometry; Mass spectroscopy; Metabolism; Microorganisms; Molecular Chaperones - metabolism; Nucleic Acids - metabolism; Oceans and Seas; Osmolar Concentration; Osmosis; Osmotic Pressure; Osmotic stress; Pathogens; Peptides; Polyamines; Potassium; Proteasomes; Protein Binding; Proteins; Proteolysis; Proteome - metabolism; Proteomes; Proteomics; Salinity; Salinity effects; Shewanella - genetics; Shewanella - metabolism; Shewanella - physiology; Shewanella baltica; Topology; Transcription, Genetic; Vibrio - genetics; Vibrio - metabolism; Vibrio - physiology; Vibrio harveyi
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23020619

Osmotic changes are common challenges for marine microorganisms. Bacteria have developed numerous ways of dealing with this stress, including reprogramming of global cellular processes. However, specific molecular adaptation mechanisms to osmotic stress have mainly been investigated in terrestrial model bacteria. In this work, we aimed to elucidate the basis of adjustment to prolonged salinity challenges at the proteome level in marine bacteria. The objects of our studies were three representatives of bacteria inhabiting various marine environments, , and The proteomic studies were performed with bacteria cultivated in increased and decreased salinity, followed by proteolytic digestion of samples which were then subjected to liquid chromatography with tandem mass spectrometry analysis. We show that bacteria adjust at all levels of their biological processes, from DNA topology through gene expression regulation and proteasome assembly, to transport and cellular metabolism. The finding that many similar adaptation strategies were observed for both low- and high-salinity conditions is particularly striking. The results show that adaptation to salinity challenge involves the accumulation of DNA-binding proteins and increased polyamine uptake. We hypothesize that their function is to coat and protect the nucleoid to counteract adverse changes in DNA topology due to ionic shifts.