Phenotypic and genotypic characterization of Pediococcus strains isolated from human clinical sources

Seventy-two strains of pediococci isolated from human clinical sources were characterized by conventional physiological tests, chromogenic enzymatic tests, analysis of whole-cell protein profiles (WCPP) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and analysis of chromosomal DNA res...

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Bibliographic Details
Published in:Journal of clinical microbiology 2001-04, Vol.39 (4), p.1241-1246
Main Authors: BARROS, Rosana R, CARVALHO, Maria Da Gloria S, PERALTA, José Mauro, FACKLAM, Richard R, TEIXEIRA, Lucia M
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Chromogenic Compounds - metabolism
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Electrophoresis, Polyacrylamide Gel
Epidemiology
Fundamental and applied biological sciences. Psychology
Genotype
Gram-Positive Bacterial Infections - microbiology
Humans
Microbiology
Pediococcus - classification
Pediococcus - genetics
Pediococcus - isolation & purification
Pediococcus - physiology
Pediococcus acidilactici
Pediococcus pentosaceus
Phenotype
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Summary:Seventy-two strains of pediococci isolated from human clinical sources were characterized by conventional physiological tests, chromogenic enzymatic tests, analysis of whole-cell protein profiles (WCPP) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and analysis of chromosomal DNA restriction profiles by pulsed-field gel electrophoresis (PFGE). Conventional tests allowed identification of 67 isolates: 52 strains were identified as Pediococcus acidilactici, 15 strains were identified as Pediococcus pentosaceus, and 5 strains were not identified because of atypical reactions. Analysis of WCPP identified all isolates since each species had a unique WCPP. By the WCPP method, the atypical strains were identified as P. acidilactici (two strains) and P. pentosaceus (three strains). The chromogenic substrate test with o-nitrophenyl-beta-D-glucopyranoside differentiated all 54 strains of P. acidilactici (negative reactions) and 13 (72%) of 18 strains of P. pentosaceus (positive reactions). Isolates of both species were shown to be nonclonal as revealed by the genetic diversity when chromosomal DNA was analyzed by PFGE. Using WCPP as the definitive identification procedure, P. acidilactici (28 of 54 strains; 51.8%) was more likely than P. pentosaceus (4 of 18 strains; 22.3%) to be isolated from blood cultures.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.39.4.1241-1246.2001