The histology of sutures in chicken skulls: Types, conservation, and ontogeny

• Published in: Journal of anatomy 2022-03, Vol.240 (3), p.503-515
• Main Authors: Arnaout, Bassel, MacKenzie, Erin M., Lantigua, Kayla E., Brzezinski, Kaylen, McKinnell, Iain W., Maddin, Hillary C.
• Format: Article
• Language: English
• Subjects: Animals; chicken; Chickens; Cranial Sutures; Developmental stages; Embryos; Endochondral bone; Histology; Mesenchyme; Ontogeny; Original Paper; Original Papers; Ossification; ossification center; Osteogenesis; Osteoid; Skull; suture; suture ontogeny; Sutures
• ISSN: 0021-8782; 1469-7580
• DOI: 10.1111/joa.13574

Sutures are fibrous joints that occur between bone elements in vertebrate skulls, where they play a variety of roles including facilitating skull growth and function. In addition, a variety of studies examining sutures from diverse perspectives in many taxa have enabled the determination of anatomical homologs. Surprisingly, one important aspect of sutures—histology—remains unknown in the key model organism of the chicken. To fill this gap in our knowledge, we generated histological sections of six different cranial sutures across a range of developmental stages in embryonic chicken. Despite having a skull that is largely co‐ossified or fused as an adult, we found that the types, components, and ontogeny of sutures in chicken skulls are very similar to sutures in other vertebrates. We did, however, find a new transient stage in the ontogeny of sutures between endochondral bone elements, in which one element has ossified and one was still cartilaginous. Moreover, to better understand the morphogenetic events at the onset of suture formation, we compared the developmental histology of six sutures with that of the space between the two ossification centers of the frontal—a location expected to be void of suture structures. We found that the mesenchymal cells in sutures condense and form a middle vascular layer. This was not found to be the case in the space between the two ossifications of the frontal, where instead only osteoid occurs. Chicken suture histology and ontogeny seem to be similar to other tetrapods. However, we have discovered a novel transient stage in the ontogeny of sutures forming between endochondral bones. We further discovered that gaps between ossification centers differ, histologically, from sutures and should not be used solely for determining homology.