Cross-Linked Gelatine by Modified Dextran as a Potential Bioink Prepared by a Simple and Non-Toxic Process

Essential features of well-designed materials intended for 3D bioprinting via microextrusion are the appropriate rheological behavior and cell-friendly environment. Despite the rapid development, few materials are utilizable as bioinks. The aim of our work was to design a novel cytocompatible materi...

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Bibliographic Details
Published in:Polymers 2022-01, Vol.14 (3), p.391
Main Authors: Musilová, Lenka, Achbergerová, Eva, Vítková, Lenka, Kolařík, Roman, Martínková, Martina, Minařík, Antonín, Mráček, Aleš, Humpolíček, Petr, Pecha, Jiří
Format: Article
Language:English
Subjects:
3-D printers
Biopolymers
Crosslinking
Dextrans
Extrusion
Fluorescence
Gelatin
Hydrogels
Inks
Molecular weight
Multilayers
NMR
Nuclear magnetic resonance
Polymerization
Reaction kinetics
Rheological properties
Rheology
Shear thinning (liquids)
Three dimensional printing
Tissue engineering
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Summary:Essential features of well-designed materials intended for 3D bioprinting via microextrusion are the appropriate rheological behavior and cell-friendly environment. Despite the rapid development, few materials are utilizable as bioinks. The aim of our work was to design a novel cytocompatible material facilitating extrusion-based 3D printing while maintaining a relatively simple and straightforward preparation process without the need for harsh chemicals or radiation. Specifically, hydrogels were prepared from gelatines coming from three sources-bovine, rabbit, and chicken-cross-linked by dextran polyaldehyde. The influence of dextran concentration on the properties of hydrogels was studied. Rheological measurements not only confirmed the strong shear-thinning behavior of prepared inks but were also used for capturing cross-linking reaction kinetics and demonstrated quick achievement of gelation point (in most cases < 3 min). Their viscoelastic properties allowed satisfactory extrusion, forming a self-supported multi-layered uniformly porous structure. All gelatin-based hydrogels were non-cytototoxic. Homogeneous cells distribution within the printed scaffold was confirmed by fluorescence confocal microscopy. In addition, no disruption of cells structure was observed. The results demonstrate the great potential of the presented hydrogels for applications related to 3D bioprinting.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym14030391