Molecular basis of a bacterial-amphibian symbiosis revealed by comparative genomics, modeling, and functional testing

• Published in: The ISME Journal 2022-03, Vol.16 (3), p.788-800
• Main Authors: Brunetti, Andrés E., Bunk, Boyke, Lyra, Mariana L., Fuzo, Carlos A., Marani, Mariela M., Spröer, Cathrin, Haddad, Célio F. B., Lopes, Norberto P., Overmann, Jörg
• Format: Article
• Language: English
• Subjects: 38/39; 45/22; 45/23; 45/43; 45/91; 631/158/855; 631/181/757; 631/208/212/2019; 631/208/212/2306; 631/326/41/547; Animals; Antifungal activity; Antiinfectives and antibacterials; Antimicrobial peptides; Anura; Bacteria; Bacteria - genetics; Biomedical and Life Sciences; Boana prasina; E coli; Ecology; Efflux; Evolutionary Biology; Functional testing; Fungicides; Gene clusters; Genome, Bacterial; Genomes; Genomics; Laboratory tests; Life Sciences; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microbiomes; Modelling; Molecular interactions; Molecular modelling; Nucleotide sequence; Peptides; Pseudomonas; Sensory evaluation; Symbiosis
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/s41396-021-01121-7

The molecular bases for the symbiosis of the amphibian skin microbiome with its host are poorly understood. Here, we used the odor-producer Pseudomonas sp. MPFS and the treefrog Boana prasina as a model to explore bacterial genome determinants and the resulting mechanisms facilitating symbiosis. Pseudomonas sp. MPFS and its closest relatives, within a new clade of the P. fluoresens Group, have large genomes and were isolated from fishes and plants, suggesting environmental plasticity. We annotated 16 biosynthetic gene clusters from the complete genome sequence of this strain, including those encoding the synthesis of compounds with known antifungal activity and of odorous methoxypyrazines that likely mediate sexual interactions in Boana prasina . Comparative genomics of Pseudomonas also revealed that Pseudomonas sp. MPFS and its closest relatives have acquired specific resistance mechanisms against host antimicrobial peptides (AMPs), specifically two extra copies of a multidrug efflux pump and the same two-component regulatory systems known to trigger adaptive resistance to AMPs in P. aeruginosa . Subsequent molecular modeling indicated that these regulatory systems interact with an AMP identified in Boana prasina through the highly acidic surfaces of the proteins comprising their sensory domains. In agreement with a symbiotic relationship and a highly selective antibacterial function, this AMP did not inhibit the growth of Pseudomonas sp. MPFS but inhibited the growth of another Pseudomonas species and Escherichia coli in laboratory tests. This study provides deeper insights into the molecular interaction of the bacteria-amphibian symbiosis and highlights the role of specific adaptive resistance toward AMPs of the hosts.