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Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing
Mouse brain slices are one of the most common models to study brain development and functioning, increasing the number of study models that integrate microfluidic systems for hippocampal slice cultures. This report presents an alternative brain slice-on-a-chip, integrating an injection system inside...
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| Published in: | International journal of molecular sciences 2022-02, Vol.23 (5), p.2549 |
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| Main Authors: | , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c478t-5b0a079ec3ba407b49eb5a9053eb0083197ad058e592bdbf750ace3e549987683 |
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| cites | cdi_FETCH-LOGICAL-c478t-5b0a079ec3ba407b49eb5a9053eb0083197ad058e592bdbf750ace3e549987683 |
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| container_issue | 5 |
| container_start_page | 2549 |
| container_title | International journal of molecular sciences |
| container_volume | 23 |
| creator | Herreros, Pedro Tapia-González, Silvia Sánchez-Olivares, Laura Laguna Heras, María Fe Holgado, Miguel |
| description | Mouse brain slices are one of the most common models to study brain development and functioning, increasing the number of study models that integrate microfluidic systems for hippocampal slice cultures. This report presents an alternative brain slice-on-a-chip, integrating an injection system inside the chip to dispense a fluorescent dye for long-term monitoring. Hippocampal slices have been cultured inside these chips, observing fluorescence signals from living cells, maintaining the cytoarchitecture of the slices. Having fluorescence images of biological samples inside the chip demonstrates the effectiveness of the staining process using the injection method avoiding leaks or biological contamination. The technology developed in this study presents a significant improvement in the local administration of reagents within a brain slice-on-a-chip system, which could be a suitable option for organotypic cultures in a microfluidic chip acting as a highly effective bioreactor. |
| doi_str_mv | 10.3390/ijms23052549 |
| format | article |
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| ispartof | International journal of molecular sciences, 2022-02, Vol.23 (5), p.2549 |
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| language | eng |
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| source | Publicly Available Content Database; PubMed |
| subjects | Animals Biological properties Bioreactors Brain Brain architecture Brain slice preparation Cell culture Contamination Fluorescence Fluorescent dyes Fluorescent indicators Hippocampus Injection Lab-On-A-Chip Devices Labeling Mice Microfluidics Organ Culture Techniques Reagents |
| title | Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing |
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