Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples

Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral...

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Bibliographic Details
Published in:PNAS nexus 2022-03, Vol.1 (1), p.pgac028
Main Authors: Breshears, Lane E, Nguyen, Brandon T, Akarapipad, Patarajarin, Sosnowski, Katelyn, Kaarj, Kattika, Quirk, Grace, Uhrlaub, Jennifer L, Nikolich-Žugich, Janko, Worobey, Michael, Yoon, Jeong-Yeol
Format: Article
Language:English
Subjects:
Detectors
Fluorescence
Fluorescence microscopy
Health aspects
Health care industry
Infection
International economic relations
Microfluidics
Physical Sciences and Engineering
Smart phones
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Summary:Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for  = 27 human subjects with  = 13 RT-qPCR positives.
ISSN:2752-6542
2752-6542
DOI:10.1093/pnasnexus/pgac028