Ac/Ds -Induced Receptor-like Kinase Genes Deletion Provides Broad-Spectrum Resistance to Bacterial Blight in Rice

Rice bacterial blight caused by pv. ( ) seriously affects rice yield production. The discovery and application of broad-spectrum resistance genes are of great advance for disease resistance breeding. Previously, we identified that multiple family gene deletions induced by the system resulted in a le...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-04, Vol.23 (9), p.4561
Main Authors: Mei, Qiong, Fu, Yu Wen, Li, Tian Miao, Xuan, Yuan Hu
Format: Article
Language:English
Subjects:
Bacteria
Blight
Chromosomes
Complementation
Crop yield
Cultivars
Disease resistance
Disease Resistance - genetics
Genes
Genetic analysis
Genomes
Genotype & phenotype
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Kinases
Lesions
Mutants
Mutation
Oryza - genetics
Oryza - microbiology
Plant Breeding
Plant Diseases - genetics
Plant Diseases - microbiology
Proteins
Reactive Oxygen Species
Xanthomonas
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Summary:Rice bacterial blight caused by pv. ( ) seriously affects rice yield production. The discovery and application of broad-spectrum resistance genes are of great advance for disease resistance breeding. Previously, we identified that multiple family gene deletions induced by the system resulted in a lesion mimic symptom. In this study, the mutant #29 showed that this lesion mimic symptom was isolated. Further analysis identified that four genes were deleted in the #29 mutant. The #29 mutant exhibited broad-spectrum resistance to and subsequent analyses identified that pathogenesis-related genes , and cellular H O levels were significantly induced in the mutant compared to wild-type plants. A genetic analysis revealed that reconstruction of , or rescued the lesion mimic symptom of the #29 mutant, indicating that these three are responsible for broad-spectrum resistance in rice. Further yeast two hybrid and bimolecular fluorescence complementation assays demonstrated that RLK20 interacts with RBOHB, which is a ROS producer in plants. Compared to wild-type plants, the #29 mutant was more, while #29/ was less, susceptible to MV (methyl-viologen), an ROS inducer. Co-expression of and reduced RBOHB-promoted H O accumulation in the cells. Taken together, our research indicated that the RLKs may inhibit RBOHB activity to negatively regulate rice resistance to . These results provide the theoretical basis and valuable information about the target genes necessary for the successful breeding of rice cultivars resistant to bacterial blight.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23094561