Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form

Objectives. The texture of the autologous platelet-rich plasma (PRP) that is used in treating degenerative joint diseases such as knee osteoarthritis (OA) is usually in liquid form. However, the turnover rate of protein metabolism in the knee synovial fluid (SF) is less than one hour. This study exa...

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Bibliographic Details
Published in:BioMed research international 2022, Vol.2022, p.6496382-9
Main Authors: Chen, Jean-Lon, Cheng, Wei-Jen, Chen, Chih-Chi, Huang, Shu-Chun, Chen, Carl P. C., Suputtitada, Areerat
Format: Article
Language:English
Subjects:
Aged
Anticoagulants
Biomedical materials
Blood
Blood platelets
Blood products
Care and treatment
Cartilage
Chambers
Chemical properties
Complex fluids
Dentifrices
Enzyme-linked immunosorbent assay
Feasibility studies
Growth factors
Heat treatment
Heating
Hematology
Humans
Joint diseases
Joints (anatomy)
Knee
Knee Joint - metabolism
Lymphocytes
Medical research
Medicine, Experimental
Molecular weight
Neutrophils
Osteoarthritis
Osteoarthritis, Knee - metabolism
Osteoarthritis, Knee - therapy
Patients
Plasma
Platelet-derived growth factor
Platelet-Derived Growth Factor - metabolism
Platelet-rich plasma
Platelet-Rich Plasma - metabolism
Protein metabolism
Protein turnover
Proteins
Proteolysis
Rehabilitation
Standard deviation
Synovial fluid
Thermal oscillations
Thermal properties
Toothpaste
Toothpastes
Turnover rate
Variance analysis
Viscosity
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Summary:Objectives. The texture of the autologous platelet-rich plasma (PRP) that is used in treating degenerative joint diseases such as knee osteoarthritis (OA) is usually in liquid form. However, the turnover rate of protein metabolism in the knee synovial fluid (SF) is less than one hour. This study examined the feasibility of the thermal oscillation technique in converting the liquid-form PRP into an injectable viscous paste-like PRP that may delay the degradation of PRP and continuously release growth factors in the knee joint for a longer period of time. Methods. This study was conducted in the rehabilitation department of a tertiary hospital. A total of 10 elderly patients with an average age of greater than 65 years and diagnosed with moderate degree of knee OA were recruited. The RegenPRP (RegenLab, Le Mont-sur-Lausanne, Switzerland) test tube chamber was used for PRP generation. A total of 60 milliliters (mL) of blood was drawn from each patient. 10 mL of blood was injected into each PRP test tube chamber. As a result, a total of 6 test tube chambers were obtained and each chamber was centrifuged for 15 minutes. Approximately 5 mL of PRP supernatant (the liquid-form end product) was aspirated and sent for thermal oscillation treatment. Five temperatures were tested: 55, 65, 75, 85, and 95 degrees Celsius. Oscillation was set at 200 revolutions per minute (rpm) for 15 minutes. The enzyme-linked immunosorbent assay (ELISA) was applied in measuring the concentration of platelet-derived growth factor (PDGF) in picogram/milliliter (pg/mL). Repeated measures ANOVA followed by the Bonferroni post hoc test was used to compare the PDGF concentrations between each testing condition. Results. Under 75 degrees Celsius of heating, the resultant paste-like PRP end product had the highest concentration of PDGF in picograms per milliliter (pg/mL) as compared with other heating conditions (p
ISSN:2314-6133
2314-6141
DOI:10.1155/2022/6496382