The gut brain in a dish: Murine primary enteric nervous system cell cultures

Background The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allo...

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Bibliographic Details
Published in:Neurogastroenterology and motility 2022-02, Vol.34 (2), p.e14215-n/a
Main Authors: Schonkeren, Simone L., Küthe, Tara T., Idris, Musa, Bon‐Frauches, Ana C., Boesmans, Werend, Melotte, Veerle
Format: Article
Language:English
Subjects:
adult mouse
Cell culture
Culture media
enteric glial cells
Enteric nervous system
enteric neurons
Gastrointestinal tract
Glial cells
Homeostasis
Nervous system
Neural networks
Neuronal-glial interactions
primary ENS culture
protocol
Review
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Summary:Background The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease. Purpose This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.
ISSN:1350-1925
1365-2982
DOI:10.1111/nmo.14215