Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana

• Published in: Plant reproduction 2022-09, Vol.35 (3), p.189-204
• Main Authors: Dupouy, Gilles, McDermott, Emma, Cashell, Ronan, Scian, Anna, McHale, Marcus, Ryder, Peter, de Groot, Joelle, Lucca, Noel, Brychkova, Galina, McKeown, Peter C., Spillane, Charles
• Format: Article
• Language: English
• Subjects: Agriculture; Arabidopsis; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biomedical and Life Sciences; Cell Biology; Chloroplasts; Complementation; CRISPR; Developmental stages; Embryo; Embryonic Development; Embryos; Gene Expression Regulation, Plant; Genomes; Life Sciences; Localization; Mutation; Original; Original Article; Peptides; Phenotypes; Plant Sciences; Plastids - metabolism; Protein C; Proteins; Redundancy; Ribosomal proteins; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; Ribosomes - genetics; Ribosomes - metabolism; Transit
• ISSN: 2194-7953; 2194-7961
• DOI: 10.1007/s00497-022-00440-9

Plastid ribosomal proteins (PRPs) can play essential roles in plastid ribosome functioning that affect plant function and development. However, the roles of many PRPs remain unknown, including elucidation of which PRPs are essential or display redundancy. Here, we report that the nuclear-encoded PLASTID RIBOSOMAL PROTEIN L5 (PRPL5) is essential for early embryo development in A. thaliana , as homozygous loss-of-function mutations in the PRPL5 gene impairs chloroplast development and leads to embryo failure to develop past the globular stage. We confirmed the prpl5 embryo-lethal phenotype by generating a mutant CRISPR/Cas9 line and by genetic complementation. As PRPL5 underwent transfer to the nuclear genome early in the evolution of Embryophyta , PRPL5 can be expected to have acquired a chloroplast transit peptide. We identify and validate the presence of an N-terminal chloroplast transit peptide, but unexpectedly also confirm the presence of a conserved and functional Nuclear Localization Signal on the protein C-terminal end. This study highlights the fundamental role of the plastid translation machinery during the early stages of embryo development in plants and raises the possibility of additional roles of plastid ribosomal proteins in the nucleus.