Role of T Lymphocyte Activation Profile in Predicting SARS-CoV-2 Severity: Experience from Tertiary Care Centre of North India

• Published in: Indian journal of hematology & blood transfusion 2023-01, Vol.39 (1), p.15-24
• Main Authors: Gupta, Anshul, Pandharipande, Archit, Gupta, Mansi, Hashim, Zia, Sanjeev, Chauhan, Priyanka, Gupta, Ruchi, Chandra, Dinesh, Singh, Manish Kumar, Kashyap, Rajesh, Rahman, Khaliqur
• Format: Article
• Language: English
• Subjects: Biomarkers; Blood Transfusion Medicine; Coronaviruses; COVID-19; Hematology; Human Genetics; Lymphocytes; Medicine; Medicine & Public Health; Oncology; Original; Original Article; Severe acute respiratory syndrome coronavirus 2
• ISSN: 0971-4502; 0974-0449; 0974-0449; 0971-4502
• DOI: 10.1007/s12288-022-01558-6

Background Immune dysregulation plays a key role in determining COVID-19 disease severity. We aimed to analyze the T cell activation profile in COVID − 19 cases and its predictive role in disease severity and outcome. Material & methods This was a prospective observational pilot study from a tertiary care COVID-19 hospital. Peripheral blood samples obtained between the fifth and seventh day of COVID-19 illness, were subjected to lymphocyte subset analysis using multicolor flowcytometry using a single tube, 8 antibodies (CD45, CD19, CD3, CD4, CD8, CD38, HLADR, and CD56) analysis. Correlation between lymphocyte subset analysis and clinical profile was determined. Results 26 patients including 11 with mild disease and 15 with severe disease were enrolled. The median age was 58 years (range: 33–81), with a male: female ratio of 1.36:1. Significant lymphopenia was observed in the severe group compared to the mild group (p