Monitoring and modulating a catalytic hybridization circuit for self-adaptive bioorthogonal DNA assembly

Constructing artificial domino nanoarchitectures, especially dynamic DNA circuits associated with the actuation of biological functions inside live cells, represents a versatile and powerful strategy to regulate the behaviors and fate of various living entities. However, the stepwise operation of co...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2022-09, Vol.13 (35), p.1428-1436
Main Authors: Gong, Xue, He, Shizhen, Li, Ruomeng, Chen, Yingying, Tan, Kaiyue, Wan, Yeqing, Liu, Xiaoqing, Wang, Fuan
Format: Article
Language:English
Subjects:
Acceleration
Actuation
Assembly
Chemistry
Circuits
Diffusion rate
Modular design
Modular systems
Monitoring
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Summary:Constructing artificial domino nanoarchitectures, especially dynamic DNA circuits associated with the actuation of biological functions inside live cells, represents a versatile and powerful strategy to regulate the behaviors and fate of various living entities. However, the stepwise operation of conventional DNA circuits always relies on freely diffusing reactants, which substantially slows down their operation rate and efficiency. Herein, a self-adaptive localized catalytic circuit (LCC) is developed to execute the self-sustained bioorthogonal assembly of DNA nanosponges within a crowded intracellular environment. The LCC-generated DNA scaffolds are utilized as versatile templates for realizing the proximity confinement of LCC reactants. Single-molecule-detecting fluorescence correlation spectroscopy (FCS) is used to explore the reaction acceleration of the catalytic circuit. This self-adaptive DNA circuit facilitates the bioorthogonal assembly of highly branched DNA networks for robust and accurate monitoring of miRNA targets. Based on its intriguing and modular design, the LCC system provides a pivotal molecular toolbox for future applications in early disease diagnosis. A localized catalytic circuit, facilitating the self-assembly of DNA nanosponges, is developed for robust and accurate monitoring of miRNA targets in live cells and mice.
ISSN:2041-6520
2041-6539
DOI:10.1039/d2sc03757b