Cellular level of coenzyme Q increases with neuronal differentiation, playing an important role in neural elongations

Deficiency of coenzyme Q has been reported in various neuro­logical diseases, and the behavior of this lipid in neurons has attracted attention. However, the behavior of this lipid in normal neurons remains unclear. In this study, we analyzed the concen­tration of coenzyme Q before and after neurona...

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Bibliographic Details
Published in:Journal of Clinical Biochemistry and Nutrition 2022, Vol.71(2), pp.89-96
Main Authors: Nakamura, Akari, Okamoto, Mizuho, Maeda, Ayaka, Jiang, Huiyu, Sugawara, Kyosuke, Kitatani, Kanae, Takekoshi, Susumu, Fujisawa, Akio, Yamamoto, Yorihiro, Kashiba, Misato
Format: Article
Language:English
Subjects:
Axonogenesis
Cell culture
Cell differentiation
Coenzyme Q
Differentiation
Growth factors
Intracellular
Lipids
Nerve growth factor
Nerves
neural differentiation
neurite outgrowth
Neurological diseases
Neurons
NGF
Original
PC12 cells
Pheochromocytoma cells
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Summary:Deficiency of coenzyme Q has been reported in various neuro­logical diseases, and the behavior of this lipid in neurons has attracted attention. However, the behavior of this lipid in normal neurons remains unclear. In this study, we analyzed the concen­tration of coenzyme Q before and after neuronal differentiation. Nerve growth factor treatment of PC12 cells caused neurite outgrowth and neuronal differentiation, and the amount of intra­cellular coenzyme Q increased dramatically during this process. In addition, when the serum was removed from the culture medium of N1E-115 cells and the neurite outgrowth was confirmed, the intracellular coenzyme Q level also increased. To elucidate the role of the increased coenzyme Q, we administered nerve growth factor to PC12 cells with coenzyme Q synthesis inhibitors and found that coenzyme Q levels decreased, neurite outgrowth was impaired, and differentiation markers were reduced. These results indicate that coenzyme Q levels increase during neuronal differentiation and that this increase is important for neurite outgrowth.
ISSN:0912-0009
1880-5086
DOI:10.3164/jcbn.21-107