Extending optical chemical tools and technologies to mice by shifting to the shortwave infrared region

Fluorescence imaging is an indispensable method for studying biological processes non-invasively in cells and transparent organisms. Extension into the shortwave infrared (SWIR, 1000–2000 nm) region of the electromagnetic spectrum has allowed for imaging in mammals with unprecedented depth and resol...

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Bibliographic Details
Published in:Current opinion in chemical biology 2022-06, Vol.68, p.102131-102131, Article 102131
Main Authors: Wong, Kelly C.Y., Sletten, Ellen M.
Format: Article
Language:English
Subjects:
Animals
Confocal microscopy
Fluorescent Dyes - chemistry
Fluorogenic probe
Fluorophore
Light-sheet microscopy
Mammals
Mice
Molecular imaging
Multicolor imaging
Multiplexed imaging
Optical Imaging - methods
Ratiometric probe
Shortwave infrared
Small molecule dye
Targeted imaging
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Summary:Fluorescence imaging is an indispensable method for studying biological processes non-invasively in cells and transparent organisms. Extension into the shortwave infrared (SWIR, 1000–2000 nm) region of the electromagnetic spectrum has allowed for imaging in mammals with unprecedented depth and resolution for optical imaging. In this review, we summarize recent advances in imaging technologies, dye scaffold modifications, and incorporation of these dyes into probes for SWIR imaging in mice. Finally, we offer an outlook on the future of SWIR detection in the field of chemical biology. [Display omitted] •Optical imaging technologies for the SWIR region enable enhanced sensitivity/resolution.•Bright SWIR contrast agents have emerged from classic fluorophore scaffolds.•Transformation of SWIR fluorophores into probes allows for analysis in living mice.
ISSN:1367-5931
1879-0402
1879-0402
DOI:10.1016/j.cbpa.2022.102131