Prolactin Action Is Necessary for Parental Behavior in Male Mice

• Published in: The Journal of neuroscience 2022-11, Vol.42 (44), p.8308-8327
• Main Authors: Smiley, Kristina O, Brown, Rosemary S E, Grattan, David R
• Format: Article
• Language: English
• Subjects: Amygdala; Animals; Brain - physiology; c-Fos protein; Circuits; Female; Forebrain; Glutamatergic transmission; Immunoreactivity; Infanticide; Juveniles; Laboratory animals; Male; Males; Maternal Behavior; Mating; Mating behavior; Mice; Neurons; Nuclei (cytology); Parental behavior; Paternal behavior; Paternal Behavior - physiology; Preoptic area; Preoptic area (medial); Preoptic Area - physiology; Prolactin; Prolactin - metabolism; Receptors, Prolactin - genetics; Receptors, Prolactin - metabolism; Stria terminalis; γ-Aminobutyric acid
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.0558-22.2022

Parental care is critical for successful reproduction in mammals. Recent work has implicated the hormone prolactin in regulating male parental behavior, similar to its established role in females. Male laboratory mice show a mating-induced suppression of infanticide (normally observed in virgins) and onset of paternal behavior 2 weeks after mating. Using this model, we sought to investigate how prolactin acts in the forebrain to regulate paternal behavior. First, using c-fos immunoreactivity in prolactin receptor (Prlr) -IRES-Cre-tdtomato reporter mouse sires, we show that the circuitry activated during paternal interactions contains prolactin-responsive neurons in multiple sites, including the medial preoptic nucleus, bed nucleus of the stria terminalis, and medial amygdala. Next, we deleted from three prominent cell types found in these regions: glutamatergic, GABAergic, and CaMKIIα. Prlr deletion from CaMKIIα, but not glutamatergic or GABAergic cells, had a profound effect on paternal behavior as none of these KO males completed the pup-retrieval task. Prolactin was increased during mating, but not in response to pups, suggesting that the mating-induced secretion of prolactin is important for establishing the switch from infanticidal to paternal behavior. Pharmacological blockade of prolactin secretion at mating, however, had no effect on paternal behavior. In contrast, suppressing prolactin secretion at the time of pup exposure resulted in failure to retrieve pups, with exogenous prolactin administration rescuing this behavior. Together, our data show that paternal behavior in sires is dependent on basal levels of circulating prolactin acting at the time of interaction with pups, mediated through Prlr on CaMKIIα-expressing neurons. Parental care is critical for offspring survival. Compared with maternal care, however, the neurobiology of paternal care is less well understood. Here we show that the hormone prolactin, which is most well known for its female-specific role in lactation, has a role in the male brain to promote paternal behavior. In the absence of prolactin signaling specifically during interactions with pups, father mice fail to show normal retrieval behavior of pups. These data demonstrate that prolactin has a similar action in both males and females to promote parental care.