Myostatin Knockout Affects Mitochondrial Function by Inhibiting the AMPK/SIRT1/PGC1α Pathway in Skeletal Muscle

• Published in: International journal of molecular sciences 2022-11, Vol.23 (22), p.13703
• Main Authors: Gu, Mingjuan, Wei, Zhuying, Wang, Xueqiao, Gao, Yang, Wang, Dong, Liu, Xuefei, Bai, Chunling, Su, Guanghua, Yang, Lei, Li, Guangpeng
• Format: Article
• Language: English
• Subjects: Acetylation; Adenosine kinase; Adenosine monophosphate; Adenosine triphosphate; Aminoimidazole Carboxamide - pharmacology; AMP-Activated Protein Kinases - metabolism; Animals; Antibodies; Biosynthesis; Body temperature; Chains; CRISPR; Dehydrogenases; Electron transport; Energy; Energy balance; Enzymes; Females; Gene deletion; Homeostasis; Isocitrate dehydrogenase; Ketoglutaric acid; Kinases; Mammals; Metabolic rate; Metabolism; Metabolites; Mice; Mice, Knockout; Microinjection; Mitochondria; Mitochondria - genetics; Mitochondria - metabolism; MSTN gene; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; Myostatin; Myostatin - genetics; Myostatin - metabolism; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism; Peroxisome proliferator-activated receptors; Phosphorylation; Protein kinase; Proteins; Rodents; Signal transduction; SIRT1 protein; Sirtuin 1 - genetics; Sirtuin 1 - metabolism; Skeletal muscle; Thermogenesis; Tricarboxylic acid cycle
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232213703

Myostatin ( ) is a major negative regulator of skeletal muscle mass and initiates multiple metabolic changes. The deletion of the gene in mice leads to reduced mitochondrial functions. However, the underlying regulatory mechanisms remain unclear. In this study, we used CRISPR/Cas9 to generate myostatin-knockout ( -KO) mice via pronuclear microinjection. -KO mice exhibited significantly larger skeletal muscles. Meanwhile, knockout regulated the organ weights of mice. Moreover, we found that knockout reduced the basal metabolic rate, muscle adenosine triphosphate (ATP) synthesis, activities of mitochondrial respiration chain complexes, tricarboxylic acid cycle (TCA) cycle, and thermogenesis. Mechanistically, expressions of silent information regulator 1 (SIRT1) and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK) were down-regulated, while peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) acetylation modification increased in the -KO mice. Skeletal muscle cells from -KO and WT were treated with AMPK activator 5-aminoimidazole-4-carboxamide riboside (AICAR), and the AMPK inhibitor Compound C, respectively. Compared with the wild-type (WT) group, Compound C treatment further down-regulated the expression or activity of pAMPK, SIRT1, citrate synthase (CS), isocitrate dehydrogenase (ICDHm), and α-ketoglutarate acid dehydrogenase (α-KGDH) in -KO mice, while knockout inhibited the AICAR activation effect. Therefore, knockout affects mitochondrial function by inhibiting the AMPK/SIRT1/PGC1α signaling pathway. The present study reveals a new mechanism for knockout in regulating energy homeostasis.