Fixing N2 into cyanophycin: continuous cultivation of Nostoc sp. PCC 7120

Two diazotrophic cyanobacteria ( Anabaena cylindrica PCC 7122 and Nostoc sp. PCC 7120) were cultivated to produce cyanophycin, a nitrogen reserve compound, under nitrogen fixing conditions. In preliminary continuous experiments, Nostoc sp. was shown to be more efficient, accumulating a higher amount...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2023-01, Vol.107 (1), p.97-110
Main Authors: Trentin, Giulia, Piazza, Francesca, Carletti, Marta, Zorin, Boris, Khozin-Goldberg, Inna, Bertucco, Alberto, Sforza, Eleonora
Format: Article
Language:English
Subjects:
Accumulation
Anabaena
Availability
Biomass
Biomedical and Life Sciences
Biotechnological Products and Process Engineering
Biotechnology
Continuous production
Cyanobacteria
Deprivation
Incident light
Life Sciences
Light intensity
Luminous intensity
Microbial Genetics and Genomics
Microbiology
Nitrogen
Nitrogen fixation
Nitrogenation
Nostoc
Phosphorus
Productivity
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Summary:Two diazotrophic cyanobacteria ( Anabaena cylindrica PCC 7122 and Nostoc sp. PCC 7120) were cultivated to produce cyanophycin, a nitrogen reserve compound, under nitrogen fixing conditions. In preliminary continuous experiments, Nostoc sp. was shown to be more efficient, accumulating a higher amount of cyanophycin and showing a greater capability to fix atmospheric nitrogen in the biomass (67 mg N d −1 of fixed nitrogen per liter of culture). The operating conditions were then optimized to maximize the cyanophycin productivity: the effect of incident light intensity, residence time and nitrogen availability were investigated. Nitrogen availability and/or pH played a major role with respect to biomass production, whereas phosphorus limitation was the main variable to maximize cyanophycin accumulation. In this way, it was possible to achieve a stable and continuous production of cyanophycin (CGP) under diazotrophic conditions, obtaining a maximum cyanophycin productivity of 15 mg CGP L −1 d −1 . Key points • Diazotrophic cyanobacteria produce stable amount of cyanophycin in continuous PBR. • Nostoc sp. proved to be more efficient in producing cyanophycin than Anabaena sp. • P deprivation is the major variable to increase cyanophycin productivity in continuous.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-022-12292-4