Plasma cytokine levels characterize disease pathogenesis and treatment response in tuberculosis patients

• Published in: Infection 2023-02, Vol.51 (1), p.169-179
• Main Authors: Vivekanandan, Monika M., Adankwah, Ernest, Aniagyei, Wilfred, Acheampong, Isaac, Yeboah, Augustine, Arthur, Joseph F., Lamptey, Millicent N. K., Abass, Mohammed K., Gawusu, Amidu, Kumbel, Francis, Osei-Yeboah, Francis, Debrah, Linda Batsa, Owusu, Dorcas O., Debrah, Alexander, Mayatepek, Ertan, Seyfarth, Julia, Phillips, Richard O., Jacobsen, Marc
• Format: Article
• Language: English
• Subjects: Bacterial infections; Biomarkers; Chemokine CXCL10; Cytokines; Family Medicine; General Practice; Granulocyte-macrophage colony-stimulating factor; Humans; Infectious Diseases; Interleukin 10; Interleukin 22; Interleukin 6; Interleukin 8; Internal Medicine; IP-10 protein; Medicine; Medicine & Public Health; Mycobacterium tuberculosis; Original Paper; Pathogenesis; Plasma; Sputum; Subgroups; Tuberculosis; Tuberculosis - drug therapy; Tuberculosis - microbiology; γ-Interferon
• ISSN: 0300-8126; 1439-0973
• DOI: 10.1007/s15010-022-01870-3

Background Mycobacterium (M.) tuberculosis -caused immunopathology is characterized by aberrant expression of plasma cytokines in human tuberculosis. Disease severity and long-term anti-mycobacterial treatment are potentially influenced by immunopathology and normalization of plasma cytokine levels during therapy may indicate treatment efficacy and recovery. Study design and methods In this study, we analyzed the concentrations of selected plasma cytokines (i.e., IL-6, IP-10, IL-10, IL-22, IFNγ, GM-CSF, IL-8) and M. tuberculosis sputum burden in patients with tuberculosis ( n  = 76). Cytokine levels were compared to healthy contacts ( n  = 40) and changes under treatment were monitored (i.e., 6 and 16 weeks after treatment start). According to differences in M. tuberculosis sputum burden and conversion, tuberculosis patients were classified as paucibacillary as well as ‘rapid’ or ‘slow’ treatment responders. A subgroup of tuberculosis patients had fatal disease courses. Results Six of seven cytokines were significantly higher in tuberculosis patients as compared to contacts and four of these (i.e., IL-6, IP-10, IL-10, and IL-22) were detectable in the majority of tuberculosis patients. IL-6 showed the strongest discriminating capacity for tuberculosis disease and in combination with IL-10 concentrations efficiently classified paucibacillary tuberculosis cases as well as those with fatal disease outcome. In addition, IL-6 and IP-10 levels decreased significantly after 6 weeks of treatment and analyses of subgroups with differential treatment response showed delayed decline of IL-6 levels in slow treatment responders. Conclusions Combinations of different plasma cytokine (namely, IL-6, IL-10, and IP-10) efficiently classified tuberculosis patients with differential mycobacterial burden and especially IL-6 qualified as a biomarker candidate for early treatment response.