Melatonin Delays Postharvest Senescence through Suppressing the Inhibition of BrERF2/BrERF109 on Flavonoid Biosynthesis in Flowering Chinese Cabbage

Flowering Chinese cabbage is prone to withering, yellowing and deterioration after harvest. Melatonin plays a remarkable role in delaying leaf senescence and increasing flavonoid biosynthesis. However, the underlying molecular mechanisms of melatonin procrastinating postharvest senescence by regulat...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-02, Vol.24 (3), p.2933
Main Authors: Yue, Lingqi, Kang, Yunyan, Zhong, Min, Kang, Dengjin, Zhao, Puyan, Chai, Xirong, Yang, Xian
Format: Article
Language:English
Subjects:
Accumulation
Aging
Antioxidants
Biosynthesis
Brassica - genetics
Brassica - metabolism
Brassica oleracea
Chinese cabbage
Chlorophyll
Enzymes
Flavonoids
Flowering
Gene Expression Regulation, Plant
Gene silencing
Leaves
Melatonin
Melatonin - metabolism
Melatonin - pharmacology
Molecular modelling
Roles
Senescence
Serotonin
Transcription factors
Yellowing
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Summary:Flowering Chinese cabbage is prone to withering, yellowing and deterioration after harvest. Melatonin plays a remarkable role in delaying leaf senescence and increasing flavonoid biosynthesis. However, the underlying molecular mechanisms of melatonin procrastinating postharvest senescence by regulating flavonoid biosynthesis remain largely unknown. In this study, melatonin could promote flavonoid accumulation and delay the postharvest senescence of flowering Chinese cabbage. Surprisingly, we observed that and were core contributors in flavonoid biosynthesis, and BrERF2 and BrERF109 were crucial ethylene response factors (ERFs) through the virus-induced gene silencing (VIGS) technique, which is involved in regulating the postharvest senescence under melatonin treatment. Furthermore, yeast one-hybrid (Y1H), dual luciferase (LUC), and β-glucuronidase (GUS) tissue staining experiments demonstrated that BrERF2/BrERF109 negatively regulated the transcripts of and by directly binding to their promoters, respectively. Silencing significantly upregulated the transcripts of and , promoting flavonoid accumulation, and postponing the leaf senescence. Our results provided a new insight into the molecular regulatory network of melatonin delaying leaf senescence and initially ascertained that melatonin promoted flavonoid accumulation by suppressing the inhibition of BrERF2/BrERF109 on the transcripts of and , which led to delaying the leaf senescence of postharvest flowering Chinese cabbage.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24032933