β-carotene improves fecal dysbiosis and intestinal dysfunctions in a mouse model of vitamin A deficiency

• Published in: Biochimica et biophysica acta. Molecular and cell biology of lipids 2022-05, Vol.1867 (5), p.159122-159122, Article 159122
• Main Authors: Honarbakhsh, Maryam, Malta, Kiana, Ericsson, Aaron, Holloway, Chelsee, Kim, Youn-Kyung, Hammerling, Ulrich, Quadro, Loredana
• Format: Article
• Language: English
• Subjects: Animals; beta Carotene - pharmacology; beta Carotene - therapeutic use; Disease Models, Animal; Dysbiosis - complications; Dysbiosis - drug therapy; Inflammation - complications; Inflammation - drug therapy; Intestinal Diseases; Intestine; Mice; Microbiome; Retinoids; Vitamin A - therapeutic use; Vitamin A deficiency; Vitamin A Deficiency - complications; Vitamin A Deficiency - drug therapy; Vitamin A Deficiency - pathology; β-carotene
• ISSN: 1388-1981; 1879-2618
• DOI: 10.1016/j.bbalip.2022.159122

Vitamin A deficiency (VAD) results in intestinal inflammation, increased redox stress and reactive oxygen species (ROS) levels, imbalanced inflammatory and immunomodulatory cytokines, compromised barrier function, and perturbations of the gut microbiome. To combat VAD dietary interventions with β-carotene, the most abundant precursor of vitamin A, are recommended. However, the impact of β-carotene on intestinal health during VAD has not been fully clarified, especially regarding the VAD-associated intestinal dysbiosis. Here we addressed this question by using Lrat−/-Rbp−/− (vitamin A deficient) mice deprived of dietary preformed vitamin A and supplemented with β-carotene as the sole source of the vitamin, alongside with WT (vitamin A sufficient) mice. We found that dietary β-carotene impacted intestinal vitamin A status, barrier integrity and inflammation in both WT and Lrat−/-Rbp−/− (vitamin A deficient) mice on the vitamin A-free diet. However, it did so to a greater extent under overt VAD. Dietary β-carotene also modified the taxonomic profile of the fecal microbiome, but only under VAD. Given the similarity of the VAD-associated intestinal phenotypes with those of several other disorders of the gut, collectively known as Inflammatory Bowel Disease (IBD) Syndrome, these findings are broadly relevant to the effort of developing diet-based intervention strategies to ameliorate intestinal pathological conditions. •Vitamin A deficiency (VAD) impairs intestinal health.•Dietary b-carotene selectively modifies the abundance of certain fecal microbial taxa and improves the dysbiosis of VAD.•Dietary b-carotene ameliorates the intestinal retinoid status and functional dysregulations of VAD.•Dietary b-carotene does not alter the gut microbiota composition under conditions of intestinal health.