Matrix metalloproteinase profiling and their roles in disease

Matrix metalloproteinases (MMPs) play roles in remodelling of the extracellular matrix that occurs during morphogenesis, repair, and angiogenesis. Dysregulation of extracellular matrix remodelling can lead to cell proliferation, invasion, and tissue fibrosis. Identification of a specific MMP(s) in a...

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Bibliographic Details
Published in:RSC advances 2023-02, Vol.13 (9), p.634-6316
Main Author: Chang, Mayland
Format: Article
Language:English
Subjects:
Affinity
Chemistry
Mass spectrometry
Matrix metalloproteinases
Resins
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Summary:Matrix metalloproteinases (MMPs) play roles in remodelling of the extracellular matrix that occurs during morphogenesis, repair, and angiogenesis. Dysregulation of extracellular matrix remodelling can lead to cell proliferation, invasion, and tissue fibrosis. Identification of a specific MMP(s) in a disease has been challenging due to the presence of 24 closely-related human MMPs, each existing in three forms, of which only one is active and capable of catalysis. This review focuses on methods for MMP profiling, with particular emphasis on the batimastat affinity resin that binds only to the active forms of MMPs and related ADAMs (a disintegrin and metalloproteinases), which are then identified by mass spectrometry. Use of the batimastat affinity resin has identified targets for intervention in several human diseases. Dysregulation of MMPs and/or ADAMs occurs in DFUs, PUs, IPF, pterygium, and GBM. The batimastat affinity resin binds only to active MMPs/ADAMs, which are identified by proteomics. Selective inhibitors are used to determine the role of the MMP/ADAM.
ISSN:2046-2069
2046-2069
DOI:10.1039/d2ra07005g