Antihyperglycemic effect of Vernonia amygdalina and in vitro evaluation of its antiproliferative activity on human osteosarcoma MG-63

• Published in: The Pan African medical journal 2022, Vol.42, p.222-222
• Main Authors: Nkono, Barnabé Lucien Nkono Ya, Rouamba, Ablassé, Duceac, Ioana Alexandra, Verestiuc, Liliana
• Format: Article
• Language: English
• ISSN: 1937-8688; 1937-8688
• DOI: 10.11604/pamj.2022.42.222.33149

the leaves of Vernonia amygdalina (V. amygdalina) are consumed as food in sub-Saharan Africa (SSA). In traditional medicine, this plant is widely used in the treatment of cancer and diabetes mellitus. In the present study, we evaluated the antihyperglycemic and the antiproliferative activities of the hydroalcoholic extract of V. amygdalina leaves (HAEVa). we conducted an experimental descriptive and analytical study with a prospective data collection from May 2019 to July 2020. For the in vivo study, the experiments were carried out on albino male rats of Wistar strain (Rattus norvegicus). Antihyperglycemic activity was performed in vivo in dexamethasone-induced insulin-resistant rats using the oral glucose tolerance test (OGTT). The biocompatibility and the antiproliferative activity of extract were performed in vitro respectively on rabbit primary dermal fibroblasts (RPDF) and human osteosarcoma MG-63 cells using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed with the GraphPad Prism software version 5.0.3. The statistical analyses were obtained by the analysis of variance (ANOVA), followed by Bonferroni´s post-test. P0.05 and p