Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis

Wnt signaling has been shown to play multiple roles in regenerative processes, one of the most widely studied of which is the regeneration of the intestinal luminal epithelia. Most studies in this area have focused on self-renewal of the luminal stem cells; however, Wnt signaling may also have more...

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Bibliographic Details
Published in:Genes 2023-01, Vol.14 (2), p.309
Main Authors: Auger, Noah A, Medina-Feliciano, Joshua G, Quispe-Parra, David J, Colón-Marrero, Stephanie, Ortiz-Zuazaga, Humberto, García-Arrarás, José E
Format: Article
Language:English
Subjects:
Animals
beta Catenin - metabolism
Cell self-renewal
Esophagus
Frizzled protein
Gene expression
Genes
Genetic aspects
Genomes
Homeostasis
Insects
Intestinal Mucosa - metabolism
Intestine
Intestines
Mesentery
Organogenesis
Physiological aspects
Signal transduction
Stem cells
Wnt protein
Wnt proteins
Wnt Signaling Pathway
β-Catenin
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Summary:Wnt signaling has been shown to play multiple roles in regenerative processes, one of the most widely studied of which is the regeneration of the intestinal luminal epithelia. Most studies in this area have focused on self-renewal of the luminal stem cells; however, Wnt signaling may also have more dynamic functions, such as facilitating intestinal organogenesis. To explore this possibility, we employed the sea cucumber that can regenerate a full intestine over the course of 21 days after evisceration. We collected RNA-seq data from various intestinal tissues and regeneration stages and used these data to define the genes present in and the differential gene expression (DGE) patterns during the regenerative process. Twelve genes were found, and their presence was confirmed in the draft genome of The expressions of additional Wnt-associated genes, such as and , as well as genes from the Wnt/β-catenin and Wnt/Planar Cell Polarity (PCP) pathways, were also analyzed. DGE showed unique distributions of Wnt in early- and late-stage intestinal regenerates, consistent with the Wnt/β-catenin pathway being upregulated during early-stages and the Wnt/PCP pathway being upregulated during late-stages. Our results demonstrate the diversity of Wnt signaling during intestinal regeneration, highlighting possible roles in adult organogenesis.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes14020309