Expanding the scope of alkyne-mediated bioconjugations utilizing unnatural amino acidsElectronic supplementary information (ESI) available: Experimental protocols, bioconjugation optimization and SDS-PAGE analysis. See DOI: 10.1039/c5cc08287k

The importance of bioconjugates within the field of chemistry drives the need for novel methodologies for their preparation. Well-defined and stable bioconjugates are easily accessible via the utilization of unnatural amino acids (UAAs). As such, we have synthesized and incorporated two new UAAs int...

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Main Authors: Maza, Johnathan C, Nimmo, Zachary M, Young, Douglas D
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Summary:The importance of bioconjugates within the field of chemistry drives the need for novel methodologies for their preparation. Well-defined and stable bioconjugates are easily accessible via the utilization of unnatural amino acids (UAAs). As such, we have synthesized and incorporated two new UAAs into green fluorescent protein, and optimized a novel Cadiot-Chodkiewicz bioconjugation, effectively expanding the toolbox of chemical reactions that can be employed in the preparation of bioconjugates. The importance of bioconjugates within the field of chemistry drives the need for novel methodologies for their preparation.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc08287k