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Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules
A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin was developed by a green protocol using solvent-free microwave irradiation. The nanohybrid was employed for concurrent load and release of silibinin and curcumin. The new...
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Published in: | RSC advances 2016-01, Vol.6 (91), p.87935-87944 |
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container_title | RSC advances |
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creator | Massaro, M Riela, S Baiamonte, C Blanco, J. L. J Giordano, C Lo Meo, P Milioto, S Noto, R Parisi, F Pizzolanti, G Lazzara, G |
description | A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin was developed by a green protocol using solvent-free microwave irradiation. The nanohybrid was employed for concurrent load and release of silibinin and curcumin. The new delivery system was characterized by means of TGA, FT-IR spectroscopy, SEM and DLS. These techniques confirm the successful loading of the two drugs in the system. SEM and DLS measurements highlighted that the nanomaterial preserves a tubular structure with an average hydrodynamic radius of
ca.
200 nm. The release of the drugs from the HNT glycocluster was investigated by means of UV-vis spectroscopy at two different pH values simulanting the typical physiological conditions of either gastric or intestinal fluids. Enzyme-linked lectin assays (ELLA) demonstrated that highly mannoside-cyclodextrins HNT entities display high affinity towards mannose selective ConA lectin. Biological assays showed that the new drug delivery system exhibits anti-proliferative activity against the investigated cell lines. Fluorescence microscopy confirmed ELLA results and it showed a high propensity of this drug delivery system to cross cell membranes and to penetrate into the cell nucleus. The results revealed that the synthesized multicavity system is a material of suitable size and nanoarchitecture to transport drugs into living cells.
A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin for delivery of natural drugs was developed. |
doi_str_mv | 10.1039/c6ra14657k |
format | article |
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ca.
200 nm. The release of the drugs from the HNT glycocluster was investigated by means of UV-vis spectroscopy at two different pH values simulanting the typical physiological conditions of either gastric or intestinal fluids. Enzyme-linked lectin assays (ELLA) demonstrated that highly mannoside-cyclodextrins HNT entities display high affinity towards mannose selective ConA lectin. Biological assays showed that the new drug delivery system exhibits anti-proliferative activity against the investigated cell lines. Fluorescence microscopy confirmed ELLA results and it showed a high propensity of this drug delivery system to cross cell membranes and to penetrate into the cell nucleus. The results revealed that the synthesized multicavity system is a material of suitable size and nanoarchitecture to transport drugs into living cells.
A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin for delivery of natural drugs was developed.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c6ra14657k</identifier><language>eng</language><subject>Assaying ; Cyclodextrins ; Drug delivery systems ; Drugs ; Lectins ; Nanostructure ; Nuclei ; Spectroscopy</subject><ispartof>RSC advances, 2016-01, Vol.6 (91), p.87935-87944</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c322t-90001ad0582ecfaab35dcdf9c67f720fe81415cfcebce54ab90ab5d8a54b83e93</citedby><cites>FETCH-LOGICAL-c322t-90001ad0582ecfaab35dcdf9c67f720fe81415cfcebce54ab90ab5d8a54b83e93</cites><orcidid>0000-0001-5089-0556</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Massaro, M</creatorcontrib><creatorcontrib>Riela, S</creatorcontrib><creatorcontrib>Baiamonte, C</creatorcontrib><creatorcontrib>Blanco, J. L. J</creatorcontrib><creatorcontrib>Giordano, C</creatorcontrib><creatorcontrib>Lo Meo, P</creatorcontrib><creatorcontrib>Milioto, S</creatorcontrib><creatorcontrib>Noto, R</creatorcontrib><creatorcontrib>Parisi, F</creatorcontrib><creatorcontrib>Pizzolanti, G</creatorcontrib><creatorcontrib>Lazzara, G</creatorcontrib><title>Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules</title><title>RSC advances</title><description>A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin was developed by a green protocol using solvent-free microwave irradiation. The nanohybrid was employed for concurrent load and release of silibinin and curcumin. The new delivery system was characterized by means of TGA, FT-IR spectroscopy, SEM and DLS. These techniques confirm the successful loading of the two drugs in the system. SEM and DLS measurements highlighted that the nanomaterial preserves a tubular structure with an average hydrodynamic radius of
ca.
200 nm. The release of the drugs from the HNT glycocluster was investigated by means of UV-vis spectroscopy at two different pH values simulanting the typical physiological conditions of either gastric or intestinal fluids. Enzyme-linked lectin assays (ELLA) demonstrated that highly mannoside-cyclodextrins HNT entities display high affinity towards mannose selective ConA lectin. Biological assays showed that the new drug delivery system exhibits anti-proliferative activity against the investigated cell lines. Fluorescence microscopy confirmed ELLA results and it showed a high propensity of this drug delivery system to cross cell membranes and to penetrate into the cell nucleus. The results revealed that the synthesized multicavity system is a material of suitable size and nanoarchitecture to transport drugs into living cells.
A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin for delivery of natural drugs was developed.</description><subject>Assaying</subject><subject>Cyclodextrins</subject><subject>Drug delivery systems</subject><subject>Drugs</subject><subject>Lectins</subject><subject>Nanostructure</subject><subject>Nuclei</subject><subject>Spectroscopy</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNpNkM1LAzEQxYMoWGov3oU9irCaZJN091jqJxYE0fOSTCbtatrUpHvY_95oRZ3LPGZ-8xgeIaeMXjJaNVegomZCyen7ARlxKlTJqWoO_-ljMknpjeZSknHFRsRe99oXNvbL0gdt0RYr7X0YUrfDYjWY2NnS6JTnSz9AAN-nHcbChVikLHW3yauIHjNTBJdPbAzbVTAdFOvgEXqP6YQcOe0TTn76mLze3rzM78vF093DfLYooeJ8Vzb5L6YtlTVHcFqbSlqwrgE1dVNOHdZMMAkO0ABKoU1DtZG21lKYusKmGpPzve82ho8e065ddwnQe73B0KeW1bVglHIhMnqxRyGGlCK6dhu7tY5Dy2j7lWY7V8-z7zQfM3y2h2OCX-4v7eoTtRp0Jg</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Massaro, M</creator><creator>Riela, S</creator><creator>Baiamonte, C</creator><creator>Blanco, J. L. J</creator><creator>Giordano, C</creator><creator>Lo Meo, P</creator><creator>Milioto, S</creator><creator>Noto, R</creator><creator>Parisi, F</creator><creator>Pizzolanti, G</creator><creator>Lazzara, G</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0001-5089-0556</orcidid></search><sort><creationdate>20160101</creationdate><title>Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules</title><author>Massaro, M ; Riela, S ; Baiamonte, C ; Blanco, J. L. J ; Giordano, C ; Lo Meo, P ; Milioto, S ; Noto, R ; Parisi, F ; Pizzolanti, G ; Lazzara, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c322t-90001ad0582ecfaab35dcdf9c67f720fe81415cfcebce54ab90ab5d8a54b83e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Assaying</topic><topic>Cyclodextrins</topic><topic>Drug delivery systems</topic><topic>Drugs</topic><topic>Lectins</topic><topic>Nanostructure</topic><topic>Nuclei</topic><topic>Spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Massaro, M</creatorcontrib><creatorcontrib>Riela, S</creatorcontrib><creatorcontrib>Baiamonte, C</creatorcontrib><creatorcontrib>Blanco, J. L. J</creatorcontrib><creatorcontrib>Giordano, C</creatorcontrib><creatorcontrib>Lo Meo, P</creatorcontrib><creatorcontrib>Milioto, S</creatorcontrib><creatorcontrib>Noto, R</creatorcontrib><creatorcontrib>Parisi, F</creatorcontrib><creatorcontrib>Pizzolanti, G</creatorcontrib><creatorcontrib>Lazzara, G</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Massaro, M</au><au>Riela, S</au><au>Baiamonte, C</au><au>Blanco, J. L. J</au><au>Giordano, C</au><au>Lo Meo, P</au><au>Milioto, S</au><au>Noto, R</au><au>Parisi, F</au><au>Pizzolanti, G</au><au>Lazzara, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules</atitle><jtitle>RSC advances</jtitle><date>2016-01-01</date><risdate>2016</risdate><volume>6</volume><issue>91</issue><spage>87935</spage><epage>87944</epage><pages>87935-87944</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin was developed by a green protocol using solvent-free microwave irradiation. The nanohybrid was employed for concurrent load and release of silibinin and curcumin. The new delivery system was characterized by means of TGA, FT-IR spectroscopy, SEM and DLS. These techniques confirm the successful loading of the two drugs in the system. SEM and DLS measurements highlighted that the nanomaterial preserves a tubular structure with an average hydrodynamic radius of
ca.
200 nm. The release of the drugs from the HNT glycocluster was investigated by means of UV-vis spectroscopy at two different pH values simulanting the typical physiological conditions of either gastric or intestinal fluids. Enzyme-linked lectin assays (ELLA) demonstrated that highly mannoside-cyclodextrins HNT entities display high affinity towards mannose selective ConA lectin. Biological assays showed that the new drug delivery system exhibits anti-proliferative activity against the investigated cell lines. Fluorescence microscopy confirmed ELLA results and it showed a high propensity of this drug delivery system to cross cell membranes and to penetrate into the cell nucleus. The results revealed that the synthesized multicavity system is a material of suitable size and nanoarchitecture to transport drugs into living cells.
A dual drug-loaded HNT-CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin for delivery of natural drugs was developed.</abstract><doi>10.1039/c6ra14657k</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5089-0556</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Assaying Cyclodextrins Drug delivery systems Drugs Lectins Nanostructure Nuclei Spectroscopy |
title | Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules |
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