An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Ribonuclease H (RNase H), an intracellular ribonuclease, plays a crucial role in cellular processes and especially relates to many disease processes. Here, we report a novel signal amplification strategy based on an RNase H-powered DNA walking machine for specific and sensitive RNase H activity dete...

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Bibliographic Details
Published in:Nanoscale 2020-01, Vol.12 (3), p.1673-1679
Main Authors: Wang, Yafang, Hu, Namin, Liu, Chang, Nie, Cunpeng, He, Manman, Zhang, Juan, Yu, Qiaoqin, Zhao, Chuan, Chen, Tingting, Chu, Xia
Format: Article
Language:English
Subjects:
Amplification
Complex systems
Deoxyribonucleic acid
DNA
Inhibitors
Nanoparticles
Ribonucleic acid
RNA
Screening
Signal processing
Strands
Walking machines
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Summary:Ribonuclease H (RNase H), an intracellular ribonuclease, plays a crucial role in cellular processes and especially relates to many disease processes. Here, we report a novel signal amplification strategy based on an RNase H-powered DNA walking machine for specific and sensitive RNase H activity detection. The DNA walking machine is composed of a small quantity of DNA walker strands and abundant FAM-labeled DNA-RNA chimeric strands on a single gold nanoparticle (AuNP). RNase H can specifically degrade the RNA fragment in a DNA-RNA hybrid duplex and trigger the autonomous movement of a DNA walker strand on the AuNP surface. During this process, each step of the walking can release the FAM-labeled RNA from the surface of the AuNP, realizing the signal amplification for RNase H sensing. This method has been successfully utilized for RNase H activity detection in a complex system and applied for screening of related inhibitors. Therefore, our RNase H-powered DNA walking machine gives a novel platform for RNase H activity detection and RNase H-associated drug discovery. A signal amplification strategy based on an RNase H-powered DNA walking machine for RNase H activity detection.
ISSN:2040-3364
2040-3372
DOI:10.1039/c9nr07550j