New small-molecule alcohol synthesis by breaking the space limitation of the "aromatic cage" in sp. AK1 BBOX

• Published in: Organic & biomolecular chemistry 2023-08, Vol.21 (31), p.6397-644
• Main Authors: Xu, Zhiqin, Mo, Yaling, Li, Zhengwen, Ban, Shurong, Song, Heng
• Format: Article
• ISSN: 1477-0520; 1477-0539
• DOI: 10.1039/d3ob00830d

Fe( ii )/2OG-dependent oxygenase γ-butyrobetaine hydroxylase (BBOX) stereoselectively hydroxylates inactive C-H bonds and produces l -carnitine. It has potential applications in the biosynthesis of l -carnitine and the synthesis of other small molecule alcohols. In this paper, we systematically explore the substrate range of Pseudomonas sp. AK1 BBOX (psBBOX), with emphasis on the quaternary ammonium portion of γ-butyrobetaine (γ-BB). The space limitation of the "aromatic cage" in psBBOX in the hydroxylation of large quaternary ammonium analogues was studied, and the role of four aromatic amino acid residues in the substrate binding mode was analyzed. Consequently, the F188A mutant was developed with the ability to hydroxylate cyclic quaternary ammonium analogues and generate new alcohol compounds by breaking the limitation of the "aromatic cage". The hydroxylation of cyclic quaternary ammonium analogues were significantly improved by semi-rational design of the "aromatic cage" amino acid in Pseudomonas sp. AK1 BBOX.