Loading…

Anomalous diffusion of under microfluidic confinement and chemical gradient

We report a two-layer microfluidic device to study the combined effect of confinement and chemical gradient on the motility of wild-type E. coli . We track individual E. coli in 50 μm and 10 μm wide microchannels, with a channel height of 2 μm, to generate quasi-2D conditions. We find that contrary...

Full description

Saved in:
Bibliographic Details
Published in:Soft matter 2023-08, Vol.19 (34), p.6446-6457
Main Authors: Raza, Md Ramiz, George, Jijo Easo, Kumari, Savita, Mitra, Mithun K, Paul, Debjani
Format: Article
Language:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report a two-layer microfluidic device to study the combined effect of confinement and chemical gradient on the motility of wild-type E. coli . We track individual E. coli in 50 μm and 10 μm wide microchannels, with a channel height of 2 μm, to generate quasi-2D conditions. We find that contrary to expectations, bacterial trajectories are superdiffusive even in the absence of a chemical (glucose) gradient. The superdiffusive behaviour becomes more pronounced upon introducing a chemical gradient or strengthening the lateral confinement. Run length distributions for weak lateral confinement in the absence of chemical gradients follow an exponential distribution. Both confinement and chemoattraction induce deviations from this behaviour, with the run length distributions approaching a power-law form under these conditions. Both confinement and chemoattraction suppress large-angle tumbles as well. Our results suggest that wild-type E. coli modulates both its runs and tumbles in a similar manner under physical confinement and chemical gradient. Our findings have implications for understanding how bacteria modulate their motility behaviour in natural habitats. We developed a two-layer microfluidic device to study the motility dynamics of E. coli . We show that both confinement and chemoattractant lead to anomalous diffusion, with power-law distributed run lengths and suppression of large-angle tumbles.
ISSN:1744-683X
1744-6848
DOI:10.1039/d3sm00286a