Candida sp in the oral cavity with and without lesions: maximal inhibitory dilution of Propolis and Periogard

• Published in: Revista de microbiologia 1999-12, Vol.30 (4), p.335-341
• Main Authors: Azevedo, Rosa Vitória Palamin(Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Análises Clínicas, Toxicológicas e Bromatológicas), Komesu, Marilena Chinalli(Universidade de São Paulo Faculdade de Odontologia de Ribeirão Preto Departamento de Estomatologia), Candido, Regina Celia(Faculdade de Ciências Farmacêuticas, Universidade de São Paulo Departamento de Análises Clínicas, Toxicológicas e Bromatológicas), Salvetti, Cristiane(Universidade de São Paulo Faculdade de Odontologia de Ribeirão Preto Departamento de Estomatologia), Rezende, Fausto Hanaoka Caetano(Universidade de São Paulo Faculdade de Odontologia de Ribeirão Preto Departamento de Estomatologia)
• Format: Article
• Language: English
• Subjects: Candidose bucal; chlorhexidine; Clorexidina; leveduras; MICROBIOLOGY; Oral candidiasis; Propolis; Própolis; yeasts
• ISSN: 0001-3714; 0001-3714
• DOI: 10.1590/S0001-37141999000400008

Fifty individuals of both sexes aged on average 45.2 years were evaluated at the Semiology Clinic of FORP-USP in order to isolate and identify yeasts from the oral cavity, with and without lesions, and to determine the maximal inhibitory dilution (MID) of the commercial products Propolis (Apis-Flora) and Periogard (Colgate) against the strains isolated. Yeasts of the genus Candida were detected in the saliva of 9/19 (47.4%) individuals with a clinically healthy mouth, 18/22 (81.8%) of individuals with oral lesions, and in 4/9 (44.4%) of patients with deviation from normality, and were detected in 19/22 (86.4%) of the lesions. In the group with oral candidiasis, we isolated in tongue and lesion, respectively for each specie: C.tropicalis (8% and 10.7%), C.glabrata (4% and 3.6%) and C.parapsilosis (2% and 3.6%), in addition to C.albicans (71.4% and 67.8%) as the only species and the prevalent. The total cfu counts/ml saliva showed a higher mean value in the group with oral candidiasis (171.5% x 10(3)) than in the control group (72.6 x 10(3)) or the group with abnormalities (8.3 x 10(3)). Most of the test strains 67/70 (95.71%) were sensitive to the antiseptics, with Propolis presenting a MID of 1:20 for 54/70/77.1%), and Periogard a MID of 1:160 for 42/70 (60%) strains from healthy sites, results similar to those obtained with strains from oral lesions. Different results were mainly observed among different species. The results indicate the possibility of using the antiseptics Propolis and Periogard (chlorhexidine) for the prevention and treatment of oral candidiasis. Foram avaliados 50 indivíduos, de ambos os sexos e faixa etária média de 42,5 anos, da clínica de Semiologia da FORP-USP, objetivando-se isolar e identificar leveduras na cavidade bucal, com e sem lesão, e determinar a DIM dos produtos comerciais Própolis (Apis-Flora) e Periogard (Colgate) frente às cepas isoladas. Leveduras do gênero Candida foram detectadas na saliva de 9(47,4%) indivíduos com boca clinicamente sadia, 18/81,8% portadores de lesões bucais e de 4/44,4% pacientes com desvio de normalidade; sendo detectadas em 19/86,4% lesões. No grupo com candidose bucal, respectivamente de língua e lesão, isolou-se C.tropicalis (8% e 10,7%), C.glabrata 4% e 3,6%) e C.parapsilosis (2% e 3,6%), além de C.albicans (71,4% e 67,8%) como espécie única e prevalente. A contagem de ufc total/ml de saliva demonstrou um valor médio no grupo com candidose bucal (171,5% x 10(3)) maior do que no controle (72