New bioemulsifiers produced by Candida lipolytica using D-glucose and babassu oil as carbon sources

• Published in: Brazilian journal of microbiology 2003-06, Vol.34 (2), p.120-123
• Main Authors: Vance-Harrop, M H, Gusmao, NBD, Campos-Takaki, GMD
• Format: Article
• Language: English
• Subjects: babassu oil; Bioemulsificante; Bioemulsifier; Candida lipolytica; MICROBIOLOGY; seawater; água do mar; óleo de babaçu
• ISSN: 1517-8382; 1678-4405; 1678-4405; 1517-8382
• DOI: 10.1590/S1517-83822003000200006

Candida lipolytica IA 1055 produced extracellular biosurfactants with emulsification activity by fermentation using babassu oil and D-glucose as carbon sources. Natural seawater diluted at 50% supplemented with urea, ammonium sulfate, and phosphate was used as economic basal medium. The best results were achieved with the YSW-B2 medium, which contained urea, ammonium sulfate, and babassu oil and with YSW-B3 medium, which contained urea, ammonium sulfate, phosphate, and babassu oil, kept under fed batch fermentation for 60 hours with 5% of babassu oil. For the two media, the maximum specific growth rates were 0.02 h-1 and 0.04 h-1; the generation times were 34.6 h-1 and 17.3 h-1, and the emulsification activities were 0.666 and 0.158 units, respectively. The molecules of these new bioemulsifiers were contituted of carbohydrates, proteins and lipids. Candida lipolytica IA 1055 produziu biosurfactantes extracelulares com atividade de emulsificação, através de fermentação utilizando óleo de babaçu e D-glicose como fontes de carbono. A água do mar diluída a 50% suplementada com uréia, sulfato de amônio e fosfato foi usada como meio basal. Os melhores resultados foram atingidos com os meios YSW-B2 (contendo uréia, sulfato de amônio e óleo de babaçu)e YSW-B3 (contendo uréia, sulfato de amônio, fosfato e óleo de babaçu), através de fermentação em batelada alimentada com 5% de óleo de babaçu. A velocidade específica de crescimento foi de 0,02 h-1 e 0,04 h-1; tempo de geração de 34,6 h-1 e 17,3 h-1 e atividade de emulsificação igual a 0,666 e 0,158 unidades, respectivamente. As moléculas dos novos bioemulsificantes demonstraram ser constituídas por carboidratos, proteínas e lipídeos.