Phosphate addition play important role on Escherichia coli cell attachment under aerobic condition

• Main Authors: Putri, Nurul Febriani, Suryadarma, Prayoga, Zakiyyah, Salsabila, Mangunwidjaja, Djumali
• Format: Conference Proceeding
• Language: English
• Subjects: Aluminum; Attachment; Bacteria; Carbon; Cultivation; Dry cells; E coli; Electrode materials; Electrodes; Glucose; Weight
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/1.5082419

The Effect of phosphate addition in cultivation media on the enhancement Escherichia coli cell attachment was investigated. Firstly, determination of electrode materials (carbon, graphite, copper, aluminum, and stainless steel) effect on cell attachment was conducted. In addition, various concentration of phosphate of 1.33, 3.32, 6.64, 9.97, 13.29, and 19.93 g/L in cultivation media was used toward cell attachment. Cell attachment of E. coli was investigated by determine the index of colonized cell, the dry cell weight, the glucose compsution, and the concetration of residual phosphate. Carbon electrodes had better E. coli cell attachment more than the other electrodes with Ics value about 0.184 and DCW value about 0.0146 g/L. The E.coli rpoS mutant strain had higher the average cell attachment more than that of parent strain with Ics value about 0.1888 and DCW value about 0.329 g/L. The increasing phosphate added induced the attachment of cells on electrode. However, at the highest concentration of phosphate, cell attachment started to have stationary conditions. It showed that the optimum phosphate addition was at 13.29 g/L with Ics value about 0.3175, Dry cell weight value about 0.02 g/L, residual phosphate concentration about 0.25 g/L, and residual glucose concentration about 2 g/L.